| Project/Area Number |
23591478
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Kochi University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKEUCHI Hiroaki 高知大学, 教育研究部医療学系, 講師 (90346560)
UCHIYAMA Jumpei 高知大学, 教育研究部医療学系, 助教 (20574619)
DAIBATA Masanori 高知大学, 教育研究部医療学系, 教授 (50263976)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | バクテリオファージ / ピロリ菌 / ファージ療法 / 吸着タンパク質 / 溶菌酵素 / 外膜 / リガンド分子 |
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| Research Abstract |
To develop a novel eradication method of Helicobater pylori using bacteriolytic activity of a bacteriophage (phage) KHP30, the morphological, physical, and genetic aspects and the life-cycle of KHP30 have been studied. The host-range of KHP30 was about 65%, suggesting that it is suitable for application use as antibacterial agent. The complete DNA nucleotide sequence was determined and the open reading frames (ORFs) were annotated. The genome consisted of 26 kbp including 30 ORFs. By homology search of the genome, the ORF for the holin protein, one component of general lytic system, was found, but the ORF for the lysin, the counterpart of the system was not found. Therefore, H. pylori phage KHP30 was supposed to have a unique bacteriolytic system different from those of other phages.
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