Project/Area Number |
23591507
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | Fukushima Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
ISE Kazuya 公立大学法人福島県立医科大学, 医学部, 講師 (90363746)
SAITO Takahiro 公立大学法人福島県立医科大学, 医学部, 博士研究員 (00566812)
|
Co-Investigator(Renkei-kenkyūsha) |
GOTOH Mitsukazu 公立大学法人福島県立医科大学, 医学部, 教授 (50162160)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
|
Keywords | 細胞シート / I型糖尿病 / 膵島移植 / 膵島再生 / ティッシュエンジニアリング / 同種移植 / 組織工学 |
Research Abstract |
Our previous study has demonstrated therapeutic effect of bioengineered islet cell sheets as a novel approach of islet transplantation for diabetes mellitus. Dispersed islet cells obtained from BALB/c mice were then plated on the laminin-5-PIPAAm dishes. After 2 days of culturing, islet cells reached confluent and were harvested as uniformly spread islet cell sheets by lowering culture temperature from 37 degrees C to 20 degrees C for 20 min. Harvested islet cell sheets were transplanted into subcutaneous site of diabetic B6 mice. Normal glucose levels were observed after 3 days of transplantation. Histological examination revealed that the transplanted islet cell sheets with MMC formed clusters of islet tissues and effect to control of inflammatory cell infiltration. It was showed that pretreatment of highly immunogenic crude pancreatic islet sheet with MMC improved their survival in allografts transplantation model.
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