Transcriptome analysis of microglia using animal model of brain diseases

Research Project

Project/Area Number	23591522
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pediatrics
Research Institution	Tokyo Metropolitan Institute of Medical Science (2012-2014) National Center of Neurology and Psychiatry (2011)
Principal Investigator	SAKUMA Hiroshi 公益財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 主席研究員 (50425683)
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000) Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords	ミクログリア / 神経炎症 / トランスクリプトーム / プリン受容体 / トランスクリプトーム解析
Outline of Final Research Achievements	Transcriptome analyses of microglia revealed that not only inflammatory but interferon- and lipid metabolism-associated genes were upregulated at the peak stage of experimental autoimmune encephalomyelitis. Using co-culture model of bone marrow immature cells and astrocytes, interleukin34 proved to be crucial for the proliferation and differentiation of microglia. Microglal adenosine A2A receptor was upregulated in the context of inflammation and negatively regulated interleukin 1β production.