The effects of neuroglycan C on neuronal network formation

• Project/Area Number: 23591524
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Institute for Developmental Research, Aichi Human Service Center
• Principal Investigator: TOKITA Yoshihito 愛知県心身障害者コロニー発達障害研究所, 周生期学部, 主任研究員 (50291175)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロテオグリカン / 向精神薬 / 増殖因子 / コンドロイチン硫酸 / 細胞増殖因子 / ニューログリカンＣ / グリコサミノグリカン / 神経

Outline of Final Research Achievements

Neuroglycan C is a brain specific chondroitin sulfate proteoglycan, which locates at dendritic spines. In this study, we revealed that Neuroglycan C interacts with some of molecules of FGF, fibroblast growth factor family. We observed that the expression levels of both Neuroglycan C and its binding partner, FGFs are enhanced by drug in the some of nucleus in the brain including the nucleus accumbens, which is known to be closely related to methamphetamine addiction. Interestingly, it has been demonstrated that some of FGFs has significant effects on presynaptic formation of both glutamatergic and GABAergic neurons. Moreover, Neuroglycan C can form molecular complex with the FGF, we thus speculate that up-regulated FGFs could be accumulated at spines with Neuroglycan C expressed on post synaptic neuronal membranes to promote neural circuit formation in these nuclei.

Research Products

• [Journal Article] Genetic Epidemiology of Tooth Agenesis in Japan: A Population-and Family-based Study. (2014)
  • Author(s): Machida J, Nishiyama T, Kishino H et al.
  • Journal Title: Clin Genet. Volume: ? Issue: 2 Pages: 1-5
  • DOI: 10.1111/cge.12456
  • Peer Reviewed
• [Journal Article] Characterization of novel MSX1 mutations identified in Japanese patients with nonsyndromic tooth agenesis (2014)
  • Author(s): Yamaguchi S, Machida J, Kamamoto M et al.
  • Journal Title: PLOSONE Volume: 9 Issue: 8 Pages: 1-9
  • DOI: 10.1371/journal.pone.0102944
  • Peer Reviewed / Open Access
• [Journal Article] A Novel PITX2 Mutation Causing Iris Hypoplasia. (2014)
  • Author(s): Kimura M, Tokita Y, Machida J. et al
  • Journal Title: Hum Genome Variation. Volume: 1 Issue: 1 Pages: 1-3
  • DOI: 10.1038/hgv.2014.5
  • Peer Reviewed / Open Access
• [Journal Article] Novel nonsense mutation in MSX1 in familial nonsyndromic oligodontia: subcellular localization and role of homeodomain/MH4. (2014)
  • Author(s): Kimura M, Machida J, Yamaguchi S. et al
  • Journal Title: Euro J Ora Sci. Volume: 122 Pages: 15-20
  • Peer Reviewed
• [Journal Article] Regeneration of Optic Nerve Fibers with Unoprostone, Prostaglandin-related Antiglaucoma Drug, in Adult Cats. (2014)
  • Author(s): Sagawa. H, Terasaki. H, Nakamura M, Nakanishi K, Tokita Y, Watanabe M.
  • Journal Title: J J Ophth Volume: 58 Pages: 100-109
  • NAID: 210000179640
  • Peer Reviewed
• [Journal Article] A novel in-frame deletion of the RUNX2 gene causes a classic form of cleidocranial dysplasia (2013)
  • Author(s): Mastushita M., Kitoh H., Kaneko H., Mishima K., Itoh Y., Tokita Y., Ishiguro N.
  • Journal Title: J Bone Miner Metab Volume: 未定 Issue: 1 Pages: 96-99
  • DOI: 10.1007/s00774-013-0456-7
  • Peer Reviewed / Open Access
• [Journal Article] Clinical and functional data implicate the Arg(151) Ser variant of MSX1 in Familial Hypodontia (2011)
  • Author(s): Kamamoto M, Machida J, Yamaguchi S, Kimura M, Ono T, Jezewski PA, Higashi Y, Nakayama A, Shimozato K, Tokita Y.
  • Journal Title: Euro J Hum Genet Volume: 19 Issue: 8 Pages: 844-50
  • DOI: 10.1038/ejhg.2011.47
  • Peer Reviewed
• [Presentation] Candidate gene analysis of non-syndromic tooth agenesis in Japanese. (2014)
  • Author(s): Machida J, Tatematsu T, Shibata A. et al.
  • Organizer: Annual meeting of American society of human genetics
  • Place of Presentation: San Diego
  • Year and Date: 2014-10-22 – 2014-10-26
• [Presentation] Novel mutation in RUNX2/CBF-alpha-1 with alanine tract expansion from Japanese cleidocranial dysplasia patient. (2014)
  • Author(s): Shibata A. Machida J, Yamaguchi S. et al.
  • Organizer: Annual meeting of American society of human genetics
  • Place of Presentation: San Diego
  • Year and Date: 2014-10-22 – 2014-10-26
• [Presentation] Novel MSX1 Mutations in Japanese Tooth Agenesis Patients. (2013)
  • Author(s): Yamaguchi S, Machida J, Kamamoto M, Kimura M, Shibata A, Tatematsu T, Miyachi H, Higashi Y, Jezewski PA, Nakayama A, Shimozato K, Tokita, Y.
  • Organizer: Annual meeting of American society of human genetics.
  • Place of Presentation: Boston (Convention & Exhibition Center)
• [Presentation] Population based study of permanent teeth agenesis in Japanese. Annual meeting of American society of human genetics. (2013)
  • Author(s): Machida J, Nishiyama T, Yamaguchi S, Kimura M, Shibata A, Tatematsu T, Abe Y, Makino S, Miyachi H, Shimozato K, Tokita Y.
  • Organizer: Annual meeting of American society of human genetics.
  • Place of Presentation: Boston (Convention & Exhibition Center)
• [Presentation] dEF1 is essential for development of the posterior lobe of pituitary. (2012)
  • Author(s): Yujiro Higashi, Tsuyoshi Takagi, Yuriko Nishizaki, Fumiko Matsui, Keiko Nakanishi, Yoshihito Tokita
  • Organizer: 日本分子生物学会大会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] Repression of Hyperactivity by Methamphetamine in Mice Deficient Neuroglycan C, a Brain-Specific Chondroitin Sulfate Proteoglycan. (2012)
  • Author(s): Aono Sachiko, Tokita Yoshihito, Matsui Fumiko, Yoneda Masahiko, Watanabe Eiji
  • Organizer: 日本神経科学会
  • Place of Presentation: 名古屋国際会議場（名古屋市）
• [Presentation] Population based study of permanent teeth agenesis in Japanese schoolchildren. (2012)
  • Author(s): J. Machida, T. Nishiyama, M. Kamamoto, S. Yamaguchi, M. Kimura, A. Shibata, K. Yamamoto, S. Makino, H. Miyachi, K. Shimozato, Y. Tokita.
  • Organizer: Annual meeting of Amecian society of human genetics.
  • Place of Presentation: Moscone Center (San Francisco)
• [Presentation] A recurrent mutation of keratin 4 gene causing white sponge nevus in a Japanese family. (2012)
  • Author(s): M. Kimura, T. Nagao, J. Machida, S. Yamaguchi, A. Shibata, G. Takeuchi, H. Miyachi, K. Shimozato, Y. Tokita.
  • Organizer: Annual meeting of Amecian society of human genetics.
  • Place of Presentation: Moscone Center (San Francisco)