Characteristics of leukemic stem cell activity in minimal residual disease of pediatric acute lymphoblastic leukemia
| Project/Area Number |
23591539
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Mie University |
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Principal Investigator |
DEGUCHI Takao 三重大学, 医学部附属病院, 講師 (70345990)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 小児血液学 / 白血病 / アポトーシス / 微小残存病変 / microRNA |
|---|
| Research Abstract |
Dexamethazone resistant acute lymphoblastic leukemia cell lines were established from sensitive cell lines. Both mRNA and protein expression of Bim showed marked reduction, and the expression of GR alpha subunit also decreased. Expressions of both miR17-92 cluster which inhibits Bim expression and miR142-3p which inhibits GR alpha expression indicated remarkable increase. Administration of mimic (agonist) or inhibitor for miR142-3p to the resistant cell lines did not indicate alteration in cell proliferation or survival. It was supposed that adequate time of mimic/inhibitor administration according to GR alpha expression should be considered, and also efficacy of mimic/inhibitor transfection should be improved. We could not get sufficient mRNA dose from freshly isolated minimal residual cells in bone marrow from leukemia patients, so still we are trying to improve the experimental procedure.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Outcome of 82 patients with TCF3-PBX1 positive pediatric acute lymphoblastic leukemia treated with JACLS ALL02 protocol2014
Author(s)
Asai D, Imamura T, Yamashita Y, Suenobu S, Saito A, Hasegawa D, Deguchi T, Hashii Y, Endo M, Hatakeyama N, Kawasaki H, Hori H, Horibe K, Yumura-Yagi K, Hara J, Watanabe A, Kikuta A, Oda M, Sato A.
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Journal Title
Cancer Med
Volume: 印刷中
Issue: 3
Pages: 623-631
DOI
Related Report
Peer Reviewed
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[Journal Article] Heterogeneity of neuroblastoma cell lines in insulin-like growth factor 1 receptor/Akt pathway-mediated cell proliferative responses2013
Author(s)
Qi L, Toyoda H, Shankar V, Sakurai N, Amano K, Kihira K, Iwasa T, Deguchi T, Hori H, Azuma E, Gabazza EC, Komada Y.
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Journal Title
Cancer Sci
Volume: 104(9)
Pages: 1162-1171
Related Report
Peer Reviewed
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[Journal Article] IKZF1 deletion is associated with a poor outcome in pediatric B-cell precursor acute lymphoblastic leukemia in Japan2013
Author(s)
Asai D, Imamura T, Suenobu S, Saito A, Hasegawa D, Deguchi T, Hashii Y, Matsumoto K, Kawasaki H, Hori H, Iguchi A, Kosaka Y, Kato K, Horibe K, Yumura-Yagi K, Hara J, Oda M.
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Journal Title
Cancer Med
Volume: 2(3)
Pages: 412-419
Related Report
Peer Reviewed
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[Journal Article] Flow cytometric analysis of de novo acute lymphoblastic leukemia in childhood : report from the Japanese Pediatric Leukemia/ Lymphoma Study Group2011
Author(s)
Iwamoto S, Deguchi T, Ohta H, Kiyokawa N, Tsurusawa M, Yamada T, Takase K, Fujimoto J, Hanada R, Hori H, Horibe K, Komada Y.
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Journal Title
Int J Hematol
Volume: 94(2)
Pages: 185-192
NAID
Related Report
Peer Reviewed
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[Journal Article] Flow cytometric analysis of de novo acute lymphoblastic leukemia in childhood: report from the Japanese Pediatric Leukemia/ Lymphoma Study Group2011
Author(s)
Iwamoto S, Deguchi T, Ohta H, Kiyokawa N, Tsurusawa M, Yamada T, Takase K, Fujimoto J, Hanada R, Hori H, Horibe K, Komada Y
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Journal Title
Int J Hematol
Volume: 94
Issue: 2
Pages: 185-92
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Outcome of 82 patients with TCF3-PBX1 positive pediatric acute lymphoblastic leukemia treated with JACLS ALL02 protocol
Author(s)
Asai D, Imamura T, Yamashita Y, Suenobu S, Saito A, Hasegawa D, Deguchi T, Hashii Y, Endo M, Hatakeyama N, Kawasaki H, Hori H, Horibe K, Yumura-Yagi K, Hara J, Watanabe A, Kikuta A, Oda M, Sato A.
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Journal Title
Cancer Med
Volume: (in press)
Related Report
Peer Reviewed
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[Presentation] Myelodysplasia-Related Changes Have Adverse Prognostic Significance in Children with Acute Myeloid Leukemia; A Report From the Japanese Pediatric Leukemia/Lymphoma Study Group (JPLSG)2012
Author(s)
Kinoshita A, Miyachi H, Matsushita H, Yabe M, Taki T, Saito A, Tomizawa D, Kiyokawa N, Taga T, Deguchi T, Hashii Y, Terui K, Takahashi H, Hayashi Y, Tawa A, Horibe K, Adachi S
Organizer
第54回アメリカ血液学会総会
URL
Place of Presentation
アトランタ
Year and Date
2012-12-09
Related Report
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[Presentation] Oda M IKZF1 deletion is associated with a poor prognosis in pediatric BCP-ALL with TCF3-PBX1 fusion2012
Author(s)
Asai D, Imamura T, Suenobu S, Saito S, Hasegawa D, Deguchi T, Hashii Y, Matsumoto K, Tokimasa S, Sato A, Iguchi A, Kosaka Y, Imaizumi M, Shima M, Hamasaki Y, Kawasaki H, Hori H, Yagi K, Horibe K, Hara J
Organizer
第74回日本血液学会学術集会
Place of Presentation
京都(臨床血液第74回日本血液学会学術集会プログラム・抄録集p337)
Year and Date
2012-10-21
Related Report
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