| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Dexamethazone resistant acute lymphoblastic leukemia cell lines were established from sensitive cell lines. Both mRNA and protein expression of Bim showed marked reduction, and the expression of GR alpha subunit also decreased. Expressions of both miR17-92 cluster which inhibits Bim expression and miR142-3p which inhibits GR alpha expression indicated remarkable increase. Administration of mimic (agonist) or inhibitor for miR142-3p to the resistant cell lines did not indicate alteration in cell proliferation or survival. It was supposed that adequate time of mimic/inhibitor administration according to GR alpha expression should be considered, and also efficacy of mimic/inhibitor transfection should be improved. We could not get sufficient mRNA dose from freshly isolated minimal residual cells in bone marrow from leukemia patients, so still we are trying to improve the experimental procedure.
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