| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia in which 25% of the patients have a mutation in the ribosomal protein S19 gene. Recently, it was reported that other ribosomal proteins (RPL5, RPL11, RPS24, RPS17) were also mutated in DBA patients. We constructed lentiviral vector expressing siRNA against RPL5, RPL11, RPS24, and RPS17 to analyze the molecular mechanism of DBA. We established cell line model of DBA after transduction with these lentiviral vector. To develop DBA model mouse, first, we generated KRAB (Kruppel-associated box) gene transgenic mouse. Using this mouse, we have a plan to make a tetracycline inducible DBA model mouse.
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