| Project/Area Number |
23591555
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fujita Health University |
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Principal Investigator |
TSUGE Ikuya 藤田保健衛生大学, 医学部, 教授 (00231431)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 経口免疫療法 / アレルゲン特異的T細胞 / 好塩基球活性化 / アレルゲン免疫療法 / CD154 / IL-4 / IL-5 / IFN-γ / Foxp3 / 低アレルゲン化食品 / 好塩基球活性化試験 / 鶏卵アレルギー |
|---|
| Research Abstract |
We analyzed the mechanism of oral immunotherapy for food allergy immunologically, with the aim to establish a safe oral immunotherapy. In this study, we evaluated allergen-specific T cells and the activation of basophils in immunotherapy using hypo-allergic hydrolyzed milk. Milk allergen specific T cells were identified as CD154 positive cells after allergen stimulation, and intracytoplasmic cytokines were simultaneously analyzed by multi-color flow-cytometry (Galios). As a result, the milk allergic patients, compared to non-milk allergic patients, milk allergen-specific Th2 cytokine producing cells have increased significantly, and milk allergen-specific IL-4 producing cells were positively correlated with milk specific IgE. On the other hand, in the analysis of basophils, the reduction of Syk involved in signal from Fc epsilon RI was observed after immunotherapy, although the number of cases is still small. It is under investigation by increasing the number of cases currently.
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