Examination of usability of passively-sensitized basophil activation after absorption test as diagnostic tool of cross-reactivity.

• Project/Area Number: 23591557
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Fujita Health University
• Principal Investigator: KONDO Yasuto 藤田保健衛生大学, 医学部, 准教授 (30301641)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 小児免疫 / アレルギー / 国際情報交換 / 食物アレルギー / 花粉症 / 口腔アレルギー症候群 / pollen food allergy / 国際情報交流

Research Abstract

Although ELISA inhibition is generally known as a method for demonstrating cross-reactivity between allergens, there are some gaps between the result and clinical phenomena.
We collected sera from the pollinosis patients with tomato-specific IgE positive, and compared the new method and the conventional method for the evaluation of the cross-reactivity between cedar and tomato. New method yields results that are closer to biological reactions in the assessment of cross-reactivity.

Research Products

• [Journal Article] Acute eosinophilic pneumonia occurring in a dedicator of cytokinesis 8 (DOCK8 ) deficient patient (2014)
  • Author(s): I Tsuge, K Ito, T Ohye, N Kando, Y Kondo, Y Nakajima, C Inuo, H Kurahashi, A Urisu.
  • Journal Title: Pediatric Pulmonology Volume: 49
  • Peer Reviewed
• [Journal Article] naphylactic reaction to dietary oats (2013)
  • Author(s): Inuo C, Kondo Y, Itagaki Y, Kurihara K, Tsuge I, Yoshikawa T, Urisu A.
  • Journal Title: Ann Allergy Asthma Immunol Volume: 110 Pages: 305-306
  • Peer Reviewed
• [Journal Article] Food sensitization in Japanese infants is associated with a common Filaggrin variant (2013)
  • Author(s): Nomura T, Tsuge I, Inuo C, Nakajima Y, Kondo Y, Sugiura S, Murata H, Iguchi T, Terada A, Saitoh S, Hashimoto S, Urisu A.
  • Journal Title: Ann Allergy Asthma Immunol Volume: 110 Pages: 388-390
  • Peer Reviewed
• [Journal Article] Japanese cedar-specific immunotherapy reduces allergen-specific Th2 cells in peripheral blood (2013)
  • Author(s): Nomura T, Tsuge I, Inuo C, Nakajima Y, Tanaka K, Naruse N, Suzuki S, Ando H, Kondo Y, Saitoh S, Urisu A.
  • Journal Title: Ann Allergy Asthma Immunol Volume: 110 Pages: 380-385
  • Peer Reviewed
• [Journal Article] A case of anaphylaxis to erythritol diagnosed by CD203c expression-based basophil activation (2013)
  • Author(s): Sugiura S, Kondo Y, Ito K, Hashiguchi A, Takeuchi M, Koyama N.
  • Journal Title: Ann Allergy Asthma Immunol Volume: 111 Pages: 222-3
  • Peer Reviewed
• [Journal Article] A case of anaphylaxis to erythritol diagnosed by CD203c expression-based basophil activation (2013)
  • Author(s): Sugiura S, Kondo Y, Ito K, Hashiguchi A, Takeuchi M, Koyama N.
  • Journal Title: Annals of Allergy Asthma & Immunology Volume: 111 Issue: 3 Pages: 222-223
  • DOI: 10.1016/j.anai.2013.06.007
  • Peer Reviewed
• [Journal Article] Anaphylactic reaction to dietary oats. (2013)
  • Author(s): Chisato Inuo
  • Journal Title: Ann Allergy Asthma Immunol. Volume: 110 Issue: 4 Pages: 305-306
  • DOI: 10.1016/j.anai.2013.01.008
  • Peer Reviewed
• [Journal Article] Food sensitization in Japanese infants is associated with a common Filaggrin variant. (2013)
  • Author(s): Nomura T, Tsuge I, Inuo C, Nakajima Y, Kondo Y, Sugiura S, Murata H, Iguchi T, Terada A, Saitoh S, Hashimoto S, Atsuo Urisu A.
  • Journal Title: Ann Allergy Asthma Immunol Volume: 110
  • Peer Reviewed
• [Journal Article] Japanese cedar-specific immunotherapy reduces allergen-specific Th2 cells in peripheral blood. (2013)
  • Author(s): Nomura T, Tsuge I, Inuo C, Nakajima Y, Tanaka K, Naruse N, Suzuki S, Ando H, Kondo Y, Saitoh S, Urisu A.
  • Journal Title: Ann Allergy Asthma Immunol Volume: 110
  • Peer Reviewed
• [Journal Article] Acute eosinophilic pneumonia occurring in a dedicator of cytokinesis 8 (DOCK8 ) deficient patient. (2013)
  • Author(s): Tsuge I, Ito K, Ohye T, Kando N, Kondo Y, deficient patient. Nakajima Y, Inuo C, Kurahashi H, Urisu A.
  • Journal Title: Pediatr Pulmonol. Volume: 48
  • Peer Reviewed
• [Journal Article] New approach for improving the safety of oral immunotherapy for food allergy (2012)
  • Author(s): Urisu A, Tanaka K, Ogura K, Naruse N, Hirata N, Nakajima Y, Inuo C, Suzuki S, Ando H, Kondo Y, Tsuge I, Yamada K, Kimura, M.
  • Journal Title: Clin Exp Allergy Rev Volume: 12 Pages: 25-28
  • Peer Reviewed
• [Journal Article] New approach for improving the safety of oral immunotherapy for food allergy. (2012)
  • Author(s): Urisu A, Tanaka K, Ogura K, Naruse N, Hirata N, Nakajima Y, Inuo C, Suzuki S, Ando H, Kondo Y, Tsuge I, Yamada K, Kimura, M.
  • Journal Title: Clin Exp Allergy Rev Volume: 12 Issue: n2 Pages: 25-28
  • DOI: 10.1111/j.1472-9733.2012.01167.x
• [Presentation] Examination of cross-reactivity using passively-sensitized basophil activation after absorption (2012)
  • Author(s): Yasuto Kondo, Kazuo Yamawaki, Yuriko Ohkubo, Kenichi Tanaka, Norihiko Naruse, Chisato Inuo, Yoichi Nakajima, Satoko Suzuki, Ikuya Tsuge, Atsuo Urisu
  • Organizer: EAACI 2012
  • Place of Presentation: Geneva, Switzerland
• [Presentation] New method for assessment of cross-reactivity (2012)
  • Author(s): Yasuto Kondo, Kenichi Tanaka, Norihiko Naruse, Satoko Suzuki, Noriko Hirata, Kazuo Yamawaki, Yuriko Ohkubo, Chisato Inuo, Yoichi Nakajima, Ikuya Tsuge, Atsuo Urisu.
  • Organizer: IPC/IOPC 2012
  • Place of Presentation: Tokyo, Japan
• [Presentation] Examination of cross-reactivity using passively-sensitized basophil activation after absorption (2012)
  • Author(s): Yasuto Kondo, Kazuo Yamawaki, Yuriko Ohkubo, Kenichi Tanaka, Norihiko Naruse, Chisato Inuo, Yoichi Nakajima, Satoko Suzuki, Ikuya Tsuge, Atsuo Urisu.
  • Organizer: European Academy of Allergy and Clinical Immunology 2012
  • Place of Presentation: Geneva
• [Presentation] New method for assessment of cross-reactivity (2012)
  • Author(s): Yasuto Kondo, Kenichi Tanaka, Norihiko Naruse, Satoko Suzuki, Noriko Hirata, Kazuo Yamawaki, Yuriko Ohkubo, Chisato Inuo, Yoichi Nakajima, Ikuya Tsuge, Atsuo Urisu.
  • Organizer: IPC/IOPC 2012
  • Place of Presentation: Tokyo
  • Invited
• [Presentation] 食物アレルゲンの交差反応性における新しい診断法の開発 (2011)
  • Author(s): 近藤康人
  • Organizer: 第18回日本免疫毒性学会
  • Place of Presentation: 千葉
  • Year and Date: 2011-09-08
• [Presentation] Passively-sensitized basophile activation is superior to ELISA inhibition for assessment of crossreactivity (2011)
  • Author(s): Yasuto Kondo, et al
  • Organizer: The 7th Congress of Asian Society for ediatric Research
  • Place of Presentation: Denver, USA
  • Year and Date: 2011-03-03
• [Presentation] Passively-sensitized basophile activation is superior to ELISA inhibition for assessment of cross-reactivity (2011)
  • Author(s): Yasuto Kondo MD, PhD, et al.
  • Organizer: The 7th Congress of Asian Society for ediatric Research
  • Place of Presentation: Denver, USA
• [Presentation] 食物アレルゲンの交差反応性における新しい診断法の開発
  • Author(s): 近藤 康人
  • Organizer: 第18回日本免疫毒性学会(招待講演)
  • Place of Presentation: 千葉大学