A diagnostic method of focal segmental glomerulosclerosis by low vacuum scanning electron microscopy

• Project/Area Number: 23591568
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Tottori University
• Principal Investigator: OKADA Shinichi 鳥取大学, 医学部, 准教授 (50343281)
• Co-Investigator(Renkei-kenkyūsha): INAGA Sumire 鳥取大学, 医学部, 助教 (60116358)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: 低真空走査型電子顕微鏡 / ネフローゼ症候群 / 糸球体上皮細胞 / 低真空型走査型電子顕微鏡

Research Abstract

We observed renal biopsy paraffin sections of nephrotic syndrome (NS) patients by low vacuum scanning electron microscopy (LVSEM). The round-shaped podocyte cell bodies were noted especially in steroid-resistant NS specimens compared with steroid-sensitive NS. The morphological changes of podocytes cell bodies may be related to the decreased expressions of nephrin or podocin in glomeruli. An application of a LVSEM to renal biopsy paraffin sections of NS patients was useful to classify focal segmental glomerulosclerosis (FSGS) specimens into the variants of the Columbia pathological classification. we considered that the evaluation method by LVSEM is probably useful the early histological diagnosis of FSGS.

Research Products

• [Journal Article] A novel approach to the histological diagnosis of pediatric nephrotic syndrome by low vacuum scanning electron microscopy (2014)
  • Author(s): Okada S, Inaga S, 他 (他7名)
  • Journal Title: Biomed Res Volume: (in press)
  • NAID: 130004678578
  • Peer Reviewed
• [Journal Article] A novel approach to the histological diagnosis of pediatric nephrotic syndrome by low vacuum scanning electron microscopy (2014)
  • Author(s): Shinichi Okada, Sumire Inaga, Yasuo Kawaba, Takuya Hanada, Atsushi Hayashi, Hironobu Nakane, Tomonori Naguro, Toshiyuki Kaidoh, Susumu Kanzaki
  • Journal Title: BiomedicalResearch Volume: in press
  • NAID: 130004678578
  • Peer Reviewed
• [Presentation] ネフローゼ症候群の病態、診断と治療 (2013)
  • Author(s): 岡田晋一
  • Organizer: 第30回中国四国小児腎臓病学会
  • Place of Presentation: 徳島市
  • Year and Date: 2013-11-17
• [Presentation] Morphological changes of podocytes in pediatric nephrotic syndrome (2013)
  • Author(s): Shinichi Okada, 他(他6名)
  • Organizer: XXIII International Symposium on Morphological Sciences
  • Place of Presentation: Niigata
  • Year and Date: 2013-09-10
• [Presentation] Morphological changes of podocytes in pediatric nephrotic syndrome (2013)
  • Author(s): Shinichi Okada, Sumire Inaga, Koichi Kitamoto, Yasuo Kawaba, Hironobu Nakane, Tomonori Naguro, Toshiyuki Kaidoh, Susumu Kanzaki
  • Organizer: XXIII International Symposium on Morphological Sciences
  • Place of Presentation: Toki Messe Niigata Convention center，Niigata，Japan
• [Presentation] 低真空走査型電子顕微鏡を用いた小児期ネフローゼ症候群腎組織の検討 (2011)
  • Author(s): 岡田晋一、稲賀すみれ, 他(他6名)
  • Organizer: 第43回日本臨床分子形態学会総会・学術集会
  • Place of Presentation: 大阪府
  • Year and Date: 2011-09-10
• [Presentation] ネフローゼ症候群の病態、診断と治療
  • Author(s): 岡田晋一
  • Organizer: 第30回 中国四国小児腎臓病学会
  • Place of Presentation: 徳島市シビックセンター、徳島市
  • Invited
• [Presentation] 低真空走査型電子顕微鏡を用いた小児期ネフローゼ症候群腎組織の検討
  • Author(s): 岡田晋一
  • Organizer: 第43回日本臨床分子形態学会
  • Place of Presentation: 大阪医科大学臨床第2講堂（大阪府）