Pan-genome analysis of virullent and antibiotics-resistant genes among Escherichia coli isolated from diarrheal children
| Project/Area Number |
23591574
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
NISHI Junichiro 鹿児島大学, 医歯(薬)学総合研究科, 教授 (40295241)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Yuichi 鹿児島大学, 大学院医歯学総合研究科, 准教授 (90237884)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASAKI Shinji 大阪府立大学, 大学院生命環境科学研究科, 教授 (70221653)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 大腸菌 / 腸管凝集性大腸菌 / ＥＳＢＬ（基質拡張型βラクタマーゼ） / 下痢症 / 小児 / 遺伝子水平伝播 / 下痢原性大腸菌 / 尿路病原性大腸菌 / ESBL（基質拡張型βラクタマーゼ） / 細胞膨化致死毒素 / ESBL |
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| Research Abstract |
A total of 6,216 E. coli strains from diarrheal children from 2001 through 2010 in our district were examined for serotyping, ESBL genotyping, and virulence genes including aggR by PCR. In addition, MLSA of ESBL-producing EAEC strains were performed. The incidence of ESBL genes in aggR-positive EAEC strains (20.8%, 31/149) was significantly higher than in other E. coli strains (1.1%, 64/6067) (OR 24.6, 95% Cl 15.5-39.3). ESBL genes were not detected in other pathotypes of diarrheagenic E. coli. All 31 ESBL-producing EAEC strains possessed CTX-M-14, most of which were found to be O25:H4:ST131. EAEC O111 and O126 strains mainly isolated since 1997 through 2010 never harbored ESBL genes, while the EAEC O25:H4:ST131 clone producing CTX-M-14 was detected in 2003 for the first time. These findings suggest that the O25:H4:ST131 clone horizontally acquired CTX-M-14 and EAEC virulence genes in our district. EAEC may play a role of reservoir of ESBL genes.
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Report
(4 results)
Research Products
(73 results)
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[Journal Article] Hyporesponsiveness to the infecting serotype after vaccination of children with seven-valent pneumococcal conjugate vaccine following invasive pneumococcal disease2014
Author(s)
Tamura K, Matsubara K, Ishiwada N, Nishi J, Ohnishi H, Suga S, Ihara T, Chang B, Akeda Y, Oishi K, Japanese I. P. D. Study Group
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Journal Title
Vaccine
Volume: 32(13)
Pages: 1444-1450
Related Report
Peer Reviewed
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[Journal Article] 嚢胞性線維症の2幼児例2014
Author(s)
柳元孝介, 西 順一郎, 田中主美, 山元公恵, 丸山慎介, 石黒 洋, 河野嘉文.
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Journal Title
日本小児呼吸器学会雑誌
Volume: 24(2)
Pages: 132-137
Related Report
Peer Reviewed
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[Journal Article] Ramsay hunt syndrome in a girl with acute lymphoblastic leukemia during maintenance therapy2013
Author(s)
Kodama Y, Okamoto Y, Nishi J, Hashiguchi S, Yamaki Y, Kurauchi K, Tanabe T, Shinkoda Y, Nishikawa T, Suda Y, Kawano Y
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Journal Title
J Pediatr Hematol Oncol
Volume: 35(5)
Related Report
Peer Reviewed
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[Journal Article] Electrocardiographic screening of 1-month-old infants for identifying prolonged QT intervals2013
Author(s)
Yoshinaga M, Ushinohama H, Sato S, Tauchi N, Horigome H, Takahashi H, Sumitomo N, Kucho Y, Shiraishi H, Nomura Y, Shimizu W, Nagashima M
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Journal Title
Circ Arrhythm Electrophysiol
Volume: 6(5)
Pages: 932-938
Related Report
Peer Reviewed
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[Journal Article] Novel cholix toxin variants, ADP-ribosylating toxins in Vibrio cholerae non-O1/non-O139 strains, and their pathogenicity2013
Author(s)
Awasthi SP, Asakura M, Chowdhury N, Neogi SB, Hinenoya A, Golbar HM, Yamate J, Arakawa E, Tada T, Ramamurthy T, Yamasaki S
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Journal Title
Infect Immun
Volume: 81(2)
Pages: 531-541
Related Report
Peer Reviewed
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[Journal Article] Ramsay Hunt Syndrome in a Girl With Acute Lymphoblastic Leukemia During Maintenance Therapy.2013
Author(s)
Kodama Y, Okamoto Y, Nishi J, Hashiguchi S, Yamaki Y, Kurauchi K, Tanabe T, Shinkoda Y, Nishikawa T, Suda Y, Kawano Y
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Journal Title
J Pediatr Hematol Oncol.
Volume: Epub ahead of print
Related Report
Peer Reviewed
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[Journal Article] Reduction in the incidence of MRSA with use of alcohol-based hand rub solutions and gloves2012
Author(s)
Matsumoto K, Shigemi A, Yaji K, Shimodozono Y, Takeda Y, Ikawa K, Morikawa N, Miyanohara H, Kawamura J, Orita M, Tokuda K, Nishi J, Yamada K
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Journal Title
J Infect Chemother
Volume: 18(2)
Pages: 269-271
NAID
Related Report
Peer Reviewed
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[Journal Article] Quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA) strains from patients with orthopaedic device-related infections2011
Author(s)
Kawamura H, Nishi J, Imuta N, Tokuda K, Miyanohara H, Hashiguchi T, Zenmyo M, Yamamoto T, Ijiri K, Kawano Y, Komiya S
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Journal Title
FEMS Immunol Med Microbiol
Volume: 63(1)
Pages: 10-15
Related Report
Peer Reviewed
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[Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms2011
Author(s)
Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T
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Journal Title
FEMS Microbiol Lett
Volume: 317(2)
Pages: 109-116
Related Report
Peer Reviewed
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[Journal Article] Reduction in the incidence of MRSA with use of alcohol-based hand rub solutions and gloves.2011
Author(s)
Matsumoto K, Shigemi A, Yaji K, Shimodozono Y, Takeda Y, Ikawa K, Morikawa N, Miyanohara H, Kawamura J, Orita M, Tokuda K, Nishi J, Yamada K
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Journal Title
J Infect Chemother
Volume: 18(2)
Issue: 2
Pages: 269-271
DOI
NAID
Related Report
Peer Reviewed
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