| Project/Area Number |
23591583
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Keio University |
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Principal Investigator |
UCHIDA KEIKO 慶應義塾大学, 医学部, 共同研究員 (50286522)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGISHI Hiroyuki 慶應義塾大学, 医学部, 准教授 (40255500)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 肺血管 / 発生 / 肺高血圧 / 発生・分化 / マイクロアレイ / 脈管形成 / 血管新生 |
|---|
| Research Abstract |
To explore the novel factors for angiogenesis of pulmonary vessels, we performed microarray analysis using RNA extracted from pulmonary endothelial cells during development of the lungs. We focused on two genes, Tbx4 and Sca-1, whose expression levels are dynamically changed during lung development, observed their expression patterns in the lung tissue using quantitative PCR and in situ hybridization, and performed in vitro tube formation assays using primary culture of the lung mesenchymal cells in order to clarify their effects on angiogenesis of the lung vessels. In addition we analyzed the roles of inositol trisphosphate receptor type 2, that is expressed in the pulmonary arteries, for pulmonary arterial hypertension, using knockout mice. Our results will provide new insight into the mechanism of exacerbation in pulmonary arterial hypertension.
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