Evaluation of oxidative stress in children with non-alcoholic steatohepatitis

• Project/Area Number: 23591589
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Osaka Medical College
• Principal Investigator: TAMAI HIROSHI 大阪医科大学, 医学部, 教授 (30179874)
• Co-Investigator(Kenkyū-buntansha): TAKITANI Kimitaka 大阪医科大学, 医学部, 講師 (80319540)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 非アルコール性脂肪性肝疾患 / 非アルコール性脂肪肝炎 / 抗酸化剤 / 脂肪性肝疾患

Research Abstract

Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in developed countries, and its incidence is increasing in adults and children. As an antioxidant, vitamin E is effective in treatment of NAFLD, although the mechanism is still unclear. Methionine-choline deficient (MCD) rats used as an experimental model of NAFLD were fed a vitamin E-enriched diet. In the MCD rats, vitamin E treatment altered the hepatic alpha-tocopherol-related protein expression, which may affect the alpha-tocopherol status in the liver and reduce lipid peroxidation.

Research Products

• [Journal Article] Theα-tocopherol status and expression ofα-tocopherol-related proteins in methionine-choline deficient rats treated with vitamin E (2014)
  • Author(s): Miyazaki H, Takitani K, Koh M, Yoden A, Tamai H
  • Journal Title: J Clin Biochem Nutr Volume: 54 Pages: 190-197
  • Peer Reviewed
• [Journal Article] Variant cardiovascular regulation in children with postural tachycardia syndrome (2014)
  • Author(s): Yoshida S, Tanaka H, Nakao R, Okamoto N, Kajiura M, Kanbara Y, Azuma S, Tamai H
  • Journal Title: Pediatr Int Volume: 56 Pages: 328-335
  • Peer Reviewed
• [Journal Article] The α-tocopherol status and expression of α-tocopherol -related proteins in methionine-choline deficient rats treated with vitamin E. (2014)
  • Author(s): Miyazaki H, Takitani K, Koh M, Yoden A, Tamai H.
  • Journal Title: J Clin Biochem Nutr. Volume: 54 Pages: 190-197
  • NAID: 130004466749
  • Peer Reviewed
• [Journal Article] Retinol status and expression of retinol-related proteins in methionine-choline deficient rats. (2014)
  • Author(s): Miyazaki H, Takitani K, Koh M, Inoue A, Kishi K, Tamai H.
  • Journal Title: J Nutr Sci Vitaminol. Volume: 60 Pages: 78-85
  • NAID: 130004491369
  • Peer Reviewed
• [Journal Article] Clinico-radiological spectrum of reversible splenial lesions in children. (2014)
  • Author(s): Kashiwagi M, Tanabe T, Shimakawa S, Nakamura M, Murata S, Shabana K, Shinohara J, Odanaka Y, Matsumura H, Koh M, Okumura K, Okasora K, Tamai H.
  • Journal Title: Brain Dev. Volume: 36 Issue: 4 Pages: 330-6
  • DOI: 10.1016/j.braindev.2013.05.007
  • Peer Reviewed
• [Journal Article] α-Tocopherol status and expression ofα-tocopherol transfer protein in type 2 diabetic Goto-Kakizaki rats (2013)
  • Author(s): Mi yazaki H, Takitani K, Koh M, Takaya R, Yoden A, Tamai H
  • Journal Title: J Nutr Sci Vitaminol Volume: 59 Pages: 64-68
  • Peer Reviewed
• [Journal Article] Association of uric acid with obesity and endothelial dysfunction in children and early adolescents (2013)
  • Author(s): Ishiro M, Takaya R, Mori Y, Takitani K, Kono Y, Okasora K, Kasahara T, Tamai H
  • Journal Title: Ann Nutr Metab Volume: 62 Pages: 169-176
  • Peer Reviewed
• [Journal Article] Liver X receptor up-regulates α-tocopherol transfer protein expression and α-tocopherol status. (2013)
  • Author(s): Koh M, Takitani K, Miyazaki H, Yamaoka S, Tamai H.
  • Journal Title: J Nutr Biochem. Volume: 24 Issue: 12 Pages: 2158-67
  • DOI: 10.1016/j.jnutbio.2013.08.008
  • Peer Reviewed
• [Journal Article] Case treated with triple therapy of lamivudine, interferon-β and prednisolone for acute exacerbation of chronic hepatitis B during pregnancy. (2013)
  • Author(s): Koh M, Shinohara J, Hongo Y, Okazaki T, Takitani K, Tamai H.
  • Journal Title: Hepatol Res. Volume: 43 Issue: 4 Pages: 425-9
  • DOI: 10.1111/j.1872-034x.2012.01077.x
  • NAID: 10031194555
  • Peer Reviewed
• [Journal Article] α-Tocopherol status and expression of α-tocopherol transfer protein in type 2 diabetic Goto-Kakizaki rats. (2013)
  • Author(s): Miyazaki H, Takitani K, Koh M, Takaya R, Yoden A, Tamai H.
  • Journal Title: J Nutr Sci Vitaminol. Volume: 59 Pages: 64-8
  • NAID: 130003368150
  • Peer Reviewed
• [Journal Article] Association of uric acid with obesity and endothelial dysfunction in children and early adolescents. (2013)
  • Author(s): Ishiro M, Takaya R, Mori Y, Takitani K, Kono Y, Okasora K, Kasahara T, Tamai H.
  • Journal Title: Ann Nutr Metab. Volume: 62 Issue: 2 Pages: 169-76
  • DOI: 10.1159/000346227
  • Peer Reviewed
• [Journal Article] Neutralization of complement component C5 ameliorates the development of dextran sulfate sodium (DSS)-colitis in mice (2013)
  • Author(s): Aomatsu T, Imaeda H, Takahashi K, Fujimoto T, Kasumi E, Ban H, Bamba S, Yoden A, Tamai H, Fujiyama Y, Andoh A.
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 52 Issue: 1 Pages: 72-75
  • DOI: 10.3164/jcbn.12-63
  • NAID: 130004879316
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] GNB3 C825T polymorphism is associated with postural tachycardia syndrome in children (2012)
  • Author(s): Nakao R, Tanaka H, Takitani K, Kajiura M, Okamoto N, Kanbara Y, Tamai H
  • Journal Title: Pediatr Int Volume: 54 Pages: 829-837
  • Peer Reviewed
• [Journal Article] Antenatal exposure to Ureaplasma species exacerbates bronchopulmonary dysplasia synergistically with subsequent prolonged mechanical ventilation in preterm infants (2012)
  • Author(s): Inatomi T, Oue S, Ogihara T, Hira S, Hasegawa M, Yamaoka S, Yasui M, Tamai H
  • Journal Title: Pediatr Res Volume: 71 Pages: 267-273
  • Peer Reviewed
• [Journal Article] Tacrolimus (FK506) suppresses TNF-α-induced CCL2 (MCP-1) and CXCL10 (IP-10) expression via the inhibition of p38 MAP kinase activation in human colonic myofibroblasts. (2012)
  • Author(s): Aomatsu T, Imaeda H, Takahashi K, Fujimoto T, Kasumi E, Yoden A, Tamai H, Fujiyama Y, Andoh A.
  • Journal Title: Int J Mol Med. Volume: 30 Issue: 5 Pages: 1152-8
  • DOI: 10.3892/ijmm.2012.1094
  • Peer Reviewed
• [Journal Article] GNB3 C825T polymorphism is associated with postural tachycardia syndrome in children. (2012)
  • Author(s): Nakao R, Tanaka H, Takitani K, Kajiura M, Okamoto N, Kanbara Y, Tamai H.
  • Journal Title: Pediatr Int. Volume: 54 Issue: 6 Pages: 829-37
  • DOI: 10.1111/j.1442-200x.2012.03707.x
  • Peer Reviewed
• [Journal Article] Terminal restriction fragment length polymorphism analysis of the gut microbiota profiles of pediatric patients with inflammatory bowel disease. (2012)
  • Author(s): Aomatsu T, Imaeda H, Fujimoto T, Takahashi K, Yoden A, Tamai H, Fujiyama Y, Andoh A.
  • Journal Title: Digestion. Volume: 86 Issue: 2 Pages: 129-35
  • DOI: 10.1159/000339777
  • Peer Reviewed
• [Journal Article] Utstein Osaka Project. Age-specific differences in outcomes after out-of-hospital cardiac arrests (2011)
  • Author(s): Nitta M, Iwami T, Kitamura T, Nadkarni VM, Berg RA, Shimizu N, Ohta K, Nishiuchi T, Hayashi Y, Hiraide A, Tamai H, Kobayashi M, Morita H
  • Journal Title: Pediatrics Volume: 128 Issue: 4 Pages: e812-e820
  • DOI: 10.1542/peds.2010-3886
  • Peer Reviewed
• [Journal Article] Induction of apoptosis byγ-tocotrienol in human cancer cell lines and leukemic blasts from patients : dependency on Bid, cytochrome c, and caspase pathway (2011)
  • Author(s): Inoue A, Takitani K, Koh M, Kawakami C, Kuno T, Tamai H
  • Journal Title: Nutr Cancer Volume: 63 Pages: 763-70
  • Peer Reviewed
• [Journal Article] Altered expression of bothβ-carotene 15,15' monooxygenase and lecithin : retinol acyltransferase in obese Zucker rats (2011)
  • Author(s): Takitani K, Miyazaki H, Fukunishi S, Takaya R, Yoden A, Higuchi K, Tamai H
  • Journal Title: J Nutr Sci Vitaminol Volume: 57 Pages: 108-13
  • NAID: 10028106443
  • Peer Reviewed
• [Journal Article] α-Tocopherol suppresses lipid peroxidation and behavioral and cognitive impairments in the Ts65Dn mouse model of Down syndrome (2011)
  • Author(s): Shichiri M, Yoshida Y, Ishida N, Hagihara Y, Iwahashi H, Tamai H, Niki E
  • Journal Title: Free Radic Biol Med Volume: 50 Pages: 1801-1811
  • Peer Reviewed
• [Journal Article] Fecal calprotectin is a useful marker for disease activity in pediatric patients with inflammatory bowel disease (2011)
  • Author(s): Aomatsu T, Yoden A, Matsumoto K, Kimura E, Inoue K, Andoh A, Tamai H
  • Journal Title: Dig Dis Sci Volume: 56 Pages: 2372-2327
  • Peer Reviewed
• [Journal Article] Meconium aspiration delays normal decline of pulmonary vascular resistance shortly after birth through lung parenchymal injury (2011)
  • Author(s): Ban R, Ogihara T, Mori Y, Oue S, Ogawa S, Tamai H
  • Journal Title: Neonatology Volume: 99 Pages: 272-9
  • Peer Reviewed
• [Journal Article] Faecal chitinase 3-like-1 : a novel biomarker of disease activity in paediatric inflammatory bowel disease (2011)
  • Author(s): Aomatsu T
  • Journal Title: Aliment Pharmacol Ther. Volume: 34 Issue: 8 Pages: 941-948
  • DOI: 10.1111/j.1365-2036.2011.04805.x
  • Peer Reviewed
• [Journal Article] Induction of apoptosis by γ-tocotrienol in human cancer cell lines and leukemic blasts from patients: dependency on Bid, cytochrome c, and caspase pathway. (2011)
  • Author(s): Inoue A, Takitani K, Koh M, Kawakami C, Kuno T, Tamai H.
  • Journal Title: Nutr Cancer. Volume: 63 Pages: 763-770
  • Peer Reviewed
• [Journal Article] Altered expression of both β-carotene 15,15' monooxygenase and lecithin:retinol acyltransferase in obese Zucker rats. (2011)
  • Author(s): Takitani K, Miyazaki H, Fukunishi S, Takaya R, Yoden A, Higuchi K, Tamai H.
  • Journal Title: J Nutr Sci Vitaminol. Volume: 57 Pages: 108-113
  • Peer Reviewed
• [Journal Article] α-Tocopherol suppresses lipid peroxidation and behavioral and cognitive impairments in the Ts65Dn mouse model of Down syndrome. (2011)
  • Author(s): Shichiri M, Yoshida Y, Ishida N, Hagihara Y, Iwahashi H, Tamai H, Niki E.
  • Journal Title: Free Radic Biol Med. Volume: 50
  • Peer Reviewed
• [Presentation] ビタミンEの臨床的意義 (2013)
  • Author(s): 玉井浩
  • Organizer: 第65回日本ビタミン学会
  • Place of Presentation: 東京
• [Presentation] ビタミンE研究の進歩 (2013)
  • Author(s): 玉井浩
  • Organizer: 第40回日本小児栄養消化器肝臓学会
• [Remarks]
  • URL: http://www.osaka-med.ac.jp/deps/ped/index.html