Development of noninvasive prenatal diagnosis using nucleated erythrocyte in maternal blood
| Project/Area Number |
23591602
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Embryonic/Neonatal medicine
|
|---|
| Research Institution | Showa University |
|---|
Principal Investigator |
MATSUOKA Ryu 昭和大学, 医学部, 講師 (20349111)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SEKIZAWA Akihiko 昭和大学, 医学部, 教授 (10245839)
YOTSUMOTO Junko 昭和大学, 医学部, 特別研究生 (30553648)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | 有核赤血球 / 出生前診断 / 無侵襲 / 無侵襲的出生前診断 / 遺伝カウンセリング / 非侵襲的出生前遺伝子診断 |
|---|
| Research Abstract |
Considerable effort has been made to develop non-invasive prenatal diagnostic procedures using nucleated erythrocytes (NRBCs) within the maternal circulation. Since a limited number of NRBCs circulate within maternal blood, procedures to enrich the cells and enable single cell analysis with high sensitivity are required. We have developed the optimized separation methods of NRBCs, including a lectin-based method and autoimage analyzing. Furthermore, we optimized every step of separation procedures to maximize the recovery.
Next, we assessed the attitudes toward non-invasive prenatal diagnosis (NIPD) in Japan. As results, we revealed that pregnant women place importance on the safety and non-invasiveness of the NIPD tests, whereas medical professionals consider the diagnostic accuracy and reliability of the test to be the most important. Health professionals pointed out that the tests might be frequently performed without the pregnant women having adequate knowledge or counseling.
|
Report
(4 results)
Research Products
(31 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Attitude toward NIPT in Japan2012
Author(s)
Yotsumoto J, Sekizawa A, Okai T
Organizer
FDD-MB WWRC 2012 (Fetal DND Diagnosis from Maternal Blood Worldwide Researchers Conference 2012)
Place of Presentation
KANAZAWA
Year and Date
2012-11-24
Related Report
-
-
-
-
-
-
-
-
