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Molecular basis of melanomagenesis based on the failure of pluripotency maintenance of melanocyte stem cells

Research Project

Project/Area Number 23591612
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionAkita University

Principal Investigator

MANABE Motomu  秋田大学, 医学(系)研究科(研究院), 教授 (30138309)

Co-Investigator(Kenkyū-buntansha) 河村 七美  聖マリアンナ医科大学, 医学部, 講師 (70323152)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsメラノーマ / スフェロイド / 三酸化ヒ素 / サリノマイシン / 幹細胞 / PI3K / STAT3 / 遺伝子改変マウス / 3次元培養法 / Stat3
Research Abstract

Recurrence after chemotherapy is a major cause of cancer mortality: subsets of tumor cells evade initial chemotherapy or radiotherapy and survive to re-propagate the tumor. To develop a novel therapeutic approach for melanoma, we applied a non-adhesive culture system which developed spheroids mimicking the properties of melanoma in vivo. Subsequently, spheroids involved cells exhibiting clonogenic and slow-cycling properties in addition to chemotherapeutic resistance to doxorubicin. Interestingly, while treatment of spheroids with either salinomycin or As2O3 showed limiting effects, a combinatorial treatment was markedly superior to single treatment with each drug. Thus, melanoma spheroids could be a new platform for studying melanoma biology and are likely to provide a clinically relevant target for the novel chemotherapy.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2013

All Journal Article (2 results) (of which Peer Reviewed: 1 results)

  • [Journal Article] Salinomycin sensitizes melanoma spheroids containing slow-cycling cells to the effects of arsenic trioxide2013

    • Author(s)
      Ishikawa N,Takahashi M,Noguchi N,Manabe M
    • Journal Title

      Akita J Med

      Volume: 40 Pages: 143-150

    • NAID

      110009795628

    • Related Report
      2013 Final Research Report
  • [Journal Article] Salinomycin sensitizes melanoma spheroids containing slow-cycling cells to the effects of arsenic trioxide.2013

    • Author(s)
      Ishikawa N,Takahashi M,Noguchi N,Manabe M.
    • Journal Title

      Akita J Med

      Volume: 40 Pages: 143-150

    • NAID

      110009795628

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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