Molecular basis of melanomagenesis based on the failure of pluripotency maintenance of melanocyte stem cells
Project/Area Number |
23591612
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Dermatology
|
Research Institution | Akita University |
Principal Investigator |
MANABE Motomu 秋田大学, 医学(系)研究科(研究院), 教授 (30138309)
|
Co-Investigator(Kenkyū-buntansha) |
河村 七美 聖マリアンナ医科大学, 医学部, 講師 (70323152)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | メラノーマ / スフェロイド / 三酸化ヒ素 / サリノマイシン / 幹細胞 / PI3K / STAT3 / 遺伝子改変マウス / 3次元培養法 / Stat3 |
Research Abstract |
Recurrence after chemotherapy is a major cause of cancer mortality: subsets of tumor cells evade initial chemotherapy or radiotherapy and survive to re-propagate the tumor. To develop a novel therapeutic approach for melanoma, we applied a non-adhesive culture system which developed spheroids mimicking the properties of melanoma in vivo. Subsequently, spheroids involved cells exhibiting clonogenic and slow-cycling properties in addition to chemotherapeutic resistance to doxorubicin. Interestingly, while treatment of spheroids with either salinomycin or As2O3 showed limiting effects, a combinatorial treatment was markedly superior to single treatment with each drug. Thus, melanoma spheroids could be a new platform for studying melanoma biology and are likely to provide a clinically relevant target for the novel chemotherapy.
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Report
(4 results)
Research Products
(2 results)