| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
Pemphigus is an intractable autoimmune blistering disease caused by IgG autoantibodies against the extracellular domains (ECs) of desmogleins (Dsgs). We have modified enzyme-linked immunosorbent assay (ELISA) using ethylenediaminetetraacetic acid (EDTA) to detect only nonpathogenic anti-Dsg3 antibodies against the non-calcium (Ca2+)-dependent epitopes. Using this method, anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes can be obtained. In this study, we examined the reliability of the ELISA-determined activities of anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes in clinical cases and demonstrated a significant correlation between the ELISA-determined activities of anti-Dsg3 serum antibodies against the Ca2+-dependent conformational epitopes and the clinical disease activities of PV patients. The conformational Dsg3 ELISA index showed improved correlation to the disease activity when monitored in individual cases.
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