Analysis of blistering mechanism in pemphigus.
| Project/Area Number |
23591620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Okayama University |
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Principal Investigator |
YUMI AOYAMA 岡山大学, 医歯(薬)学総合研究科, 准教授 (90291393)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 天疱瘡 / 自己免疫 / ケラチノサイト / 自己免疫性水疱症 / 細胞内シグナル伝達経路 / 尋常性天疱瘡 / 落葉状天疱瘡 / 病態 / 治療 / 診断 / デスモグレイン / デスモソーム / 自己抗体 |
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| Research Abstract |
Pemphigus is an intractable autoimmune blistering disease caused by IgG autoantibodies against the extracellular domains (ECs) of desmogleins (Dsgs). We have modified enzyme-linked immunosorbent assay (ELISA) using ethylenediaminetetraacetic acid (EDTA) to detect only nonpathogenic anti-Dsg3 antibodies against the non-calcium (Ca2+)-dependent epitopes. Using this method, anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes can be obtained. In this study, we examined the reliability of the ELISA-determined activities of anti-Dsg3 serum antibodies against the Ca2+-dependent epitopes in clinical cases and demonstrated a significant correlation between the ELISA-determined activities of anti-Dsg3 serum antibodies against the Ca2+-dependent conformational epitopes and the clinical disease activities of PV patients. The conformational Dsg3 ELISA index showed improved correlation to the disease activity when monitored in individual cases.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Detection of Autoantibodies to Precursor Proteins of Desmogleins in Sera of a Patient with Bowen Carcinoma2014
Author(s)
Demitsu T, Yamada T, Nakamura S, Kakurai M, Dohmoto T, Kamiya K, Aoyama Y, Iwatsuki K, Yamagami J, Ohyama B, Ohata C, Koga H, Hashimoto T
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Journal Title
Acta Derm Venereol
Volume: 94
Issue: 5
Pages: 601-603
DOI
Related Report
Peer Reviewed
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