| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
We have reported that increase of allergen absorption is one of factors which enhance the allergic symptoms in patients with wheat-dependent exercise-induced anaphylaxis. In this study, we investigate the method to evaluate the allergenicity of peripheral blood food allergen by beta-hexosaminidase assay in rat basophil leukemia cells (RBL-2H3). Degranulation of RBL-2H3 cells sensitized with the sera from Brown Norway rat having allergen specific-IgE was observed, suggesting that the biological activity of allergen in the blood could be estimated by using the method. In addition, we identified gamma-gliadin as a major allergen and its IgE-binding epitope in WDEIA induced by a soap containing hydrolyzed wheat.
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