Development of a quantification method for peripheral blood wheat allergen and its clinical application
| Project/Area Number |
23591621
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Hiroshima University |
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Principal Investigator |
MATSUO HIROAKI 広島大学, 医歯薬保健学研究院, 教授 (60346385)
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| Co-Investigator(Kenkyū-buntansha) |
MIHARA Shoji 広島大学, 大学院医歯薬保健学研究院, 准教授 (90325168)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 食物アレルギー / アレルゲン / 小麦 / 消化管透過性 / 小麦アレルゲン / 血中濃度 / グリアジン |
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| Research Abstract |
We have reported that increase of allergen absorption is one of factors which enhance the allergic symptoms in patients with wheat-dependent exercise-induced anaphylaxis. In this study, we investigate the method to evaluate the allergenicity of peripheral blood food allergen by beta-hexosaminidase assay in rat basophil leukemia cells (RBL-2H3). Degranulation of RBL-2H3 cells sensitized with the sera from Brown Norway rat having allergen specific-IgE was observed, suggesting that the biological activity of allergen in the blood could be estimated by using the method. In addition, we identified gamma-gliadin as a major allergen and its IgE-binding epitope in WDEIA induced by a soap containing hydrolyzed wheat.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Aspirin Augments IgE-Mediated Histamine Release from Human Peripheral Basophils via Syk Kinase Activation2013
Author(s)
Matsuo H, Yokooji T, Morita H, Ooi M, Urata K, Ishii K, Takahagi S, Yanase Y, Hiragun T, Mihara S, Hide M
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Journal Title
Allergology International
Volume: 62
Issue: 4
Pages: 503-511
DOI
NAID
ISSN
1323-8930, 1440-1592
Related Report
Peer Reviewed
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[Journal Article] Characterization of Causative Allergens for Wheat-Dependent Exercise-Induced Anaphylaxis Sensitized with Hydrolyzed Wheat Proteins in Facial Soap2013
Author(s)
Yokooji T, Kurihara S, Murakami T, Chinuki Y, Takahashi H, Morita E, Harada S, Ishii K, Hiragun M, Hide M, Matsuo H
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Journal Title
Allergology International
Volume: 62
Issue: 4
Pages: 435-445
DOI
NAID
ISSN
1323-8930, 1440-1592
Related Report
Peer Reviewed
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[Journal Article] Serum Gliadin Monitoring Extracts Patients with False Negative Results in Challenge Tests for the Diagnosis of Wheat-Dependent Exercise-Induced Anaphylaxis2013
Author(s)
Kohno K, Matsuo H, Takahashi H, Niihara H, Chinuki Y, Kaneko S, Honjoh T, Horikawa T, Mihara S, Morita E
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Journal Title
Allergology International
Volume: 62
Issue: 2
Pages: 229-238
DOI
NAID
ISSN
1323-8930, 1440-1592
Related Report
Peer Reviewed
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[Presentation] Characterization of the Causative Allergens for Wheat-Dependent Exercise-Induced Anaphylaxis Sensitized with Hydrolyzed Wheat Proteins in Facial Soap2013
Author(s)
Yokooji T, Kurihara S, Murakami T, Chinuki Y, Harada S, Takahhi H, Morita E, Ishii K, Hiragun M, Hide M and Matsuo H
Organizer
2013 American Academy of Allergy, Asthma & Immunology Annual Meeting
Place of Presentation
San Antonio, Texas, USA
Related Report
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[Presentation] Characterization of the Causative Allergens for Wheat-Dependent Exercise-Induced Anaphylaxis Sensitized with Hydrolyzed Wheat Proteins in Facial Soap2013
Author(s)
Tomoharu Yokooji, Saki Kurihara, Tomoko Murakami, Yuko Chinuki, Susumu Harada, Hitoshi Takahhi, Eishin Morita, Kaori Ishii, Makiko Hiragun, Michihiro Hide and Hiroaki Matsuo
Organizer
2013 American Academy of Allergy, Asthma & Immunology Annual Meeting, San Antonio, Texas
Place of Presentation
San Antonio, USA
Related Report
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