| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
The pathomechanism of the formation of bullae in bullous pemphigoid has been considered to be caused by 1) autoantibody (BP-IgG)-autoantigen (BP180) adhesion, 2) activation of complement, 3) migration of neutrophils and 4) proteolysis by neutrophil elastase. In this study, we performed live cell imaging of BP180-GFP after BP-IgG treatment on keratinocytes. The results suggested that BP180 was incorporated by macropinocytic endocytosis pathway, and that the keratinocyte cell adhesion was weakened by this pathway. We consider that this newly found initial pathomechanism of bullous pemphigoid may be the future drug target. We will be able to inhibit macropinocytosis to cure bullous pemphigoid after the drug discovery affecting it.
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