The cAMP elevation and CREB activation stimulates DNA repair capacity in skin and neuronal cells.

• Project/Area Number: 23591626
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Nara Medical University
• Principal Investigator: KOBAYASHI Nobuhiko 奈良県立医科大学, 医学部, 准教授 (70316074)
• Co-Investigator(Kenkyū-buntansha): MORI Toshio 奈良県立医科大学, 医学部, 研究教授 (10115280)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: DBcAMP / 神経芽細胞腫 / DNA修復 / サイクロプリン / モノクローナル抗体 / 紫外線感受性

Research Abstract

A basic research was performed to develop a novel method in preventing sun-induced skin cancers and melanomas, and progressive neurological disease in xeroderma pigmentosum (XP) patients using agents that can stimulate DNA repair capacity through the elevation of cellular cAMP level and CREB activity. Our results suggested that DBcAMP can stimulate DNA repair capacity in neuroblastoma cells.
Cyclopurines are candidates to be involved with XP neurological disease. Thus, we established a novel monoclonal antibody specific for a type of cyclopurine, cyclo-dA, in order to determine whether DBcAMP can also stimulate repair capacity for cyclopurines.

Research Products

• [Journal Article] Quantitative and in situ detection of oxidatively generated DNA damage 8,5’-cyclo-2’-deoxyadenosine using an immunoassay with a novel monoclonal antibody (2014)
  • Author(s): T. Iwamoto, P.J. Brooks, T. Nishiwaki, K. Nishimura, N. Kobayashi, S. Sugiura and T. Mori.
  • Journal Title: Photochem. Photobiol. Volume: xx Issue: 4 Pages: 829-836
  • DOI: 10.1111/php.12239
  • Peer Reviewed / Open Access
• [Journal Article] Characterization of three XPG-defective patients identifies three missense mutations that impair repair and transcription. (2013)
  • Author(s): A. Schafer, S. Schubert, A. Gratchev, C. Seebode, A. Apel, P. Laspe, L. Hofmann, A. Ohlenbusch, T. Mori, N. Kobayashi, A. Schurer, M.P. Schon, S. Emmert.
  • Journal Title: J. Invest. Dermatol. Volume: 133 Issue: 7 Pages: 1841-1849
  • DOI: 10.1038/jid.2013.54
  • Peer Reviewed
• [Presentation] Quantitative and in situ detection of oxidatively generated DNA damage 8,5'-cyclo-2'-deoxyadenosine using an immunoassay with a novel monoclonal antibody (2014)
  • Author(s): Iwamoto T, Brooks PJ, Kobayashi N, Sugiura S, Mori T
  • Organizer: International symposium on xeroderma pigmentosum and related diseases : disorders of DNA damage response–bench to bedside-
  • Place of Presentation: Kobe
• [Presentation] Quantitative and in situ detection of oxidatively generated DNA damage 8,5’-cyclo-2’-deoxyadenosine using an immunoassay with a novel monoclonal antibody. (2014)
  • Author(s): Iwamoto T, Brooks PJ, Kobayashi N, Sugiura S, Mori T
  • Organizer: International symposium on xeroderma pigmentosum and related diseases: disorders of DNA damage response -bench to bedside-
  • Place of Presentation: Kobe
• [Presentation] 太陽紫外線による3主要ピリミジン2量体DNA損傷の誘発 (2011)
  • Author(s): 森俊雄、吉田唯真、西村和樹、岩本顕聡、池畑広伸、小林信彦
  • Organizer: 第33回日本光医学・光生物学会
  • Place of Presentation: 吹田市
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~derm/
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~derm/gyouseki.html
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~ri/Publication2011.pdf