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detailed characterization of autoantibodies in paraneoplastic pemphigus by phage display

Research Project

Project/Area Number 23591628
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionToho University (2012-2013)
Teikyo University (2011)

Principal Investigator

ISHII Ken  東邦大学, 医学部, 准教授 (50296670)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords皮膚科学 / 自己免疫性疾患 / 細胞接着 / 自己免疫 / 天疱瘡 / 自己抗体
Research Abstract

To study the pathogenic mechanism of pareneoplastic pemphigus(PNP), we used phage display to clone monoclonal anti-Dsg3 antibodies from a PNP patient. We isolated 20 unique Dsg3-reactive mAbs, which we classified into four groups according to amino acid sequence. Pathogenic assay showed that three antibodies displayed pathogenic activity in blister formation with different potencies. Epitope mapping showed that these pathogenic mAbs bound Ca(2+)-dependent conformational epitopes in the middle portion of the extracellular region of Dsg3. These mAbs reflect the unique polyclonal nature of anti-Dsg3 antibodies in PNP and represent an important tool for detailing the pathophysiological mechanisms of blister formation in PNP.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results) Book (1 results)

  • [Journal Article] Sporadic VACTERL association in a Japanese family with Sjogren-Larsson syndrome.2013

    • Author(s)
      Takeichi T, Sugiura K, Arai H, Ishii K, Kono M, Akiyama M.
    • Journal Title

      Acta Dermato-Venereol

      Volume: 93 Issue: 5 Pages: 579-580

    • DOI

      10.2340/00015555-1526

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional tight junction barrier localizes in the second layer of the stratum granulosum of human epidermis2013

    • Author(s)
      Yoshida K, Yokouchi M, Nagao K, Ishii K, Amagai M, Kubo A
    • Journal Title

      J Dermatol Sci

      Volume: 71 Issue: 2 Pages: 89-99

    • DOI

      10.1016/j.jdermsci.2013.04.021

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus2013

    • Author(s)
      Ishii, K.
    • Journal Title

      Methods Mol Biol

      Volume: 961 Pages: 219-225

    • DOI

      10.1007/978-1-62703-227-8_13

    • ISBN
      9781627032261, 9781627032278
    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pathogenic anti-desmoglein 3 mAbs cloned from a paraneoplastic pemphigus patient by phage display2012

    • Author(s)
      Saleh MA, Ishii K, Yamagami J, Shirakata Y, Hashimoto K, Amagai M
    • Journal Title

      J Invest Dermatol

      Volume: 132(4) Issue: 4 Pages: 1141-1148

    • DOI

      10.1038/jid.2011.449

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Development of NC1 and NC2 domains of type VII collagen ELISA for the diagnosis and analysis of the time course of epidermolysis bullosa acquisita patients2011

    • Author(s)
      Saleh MA
    • Journal Title

      J Dermatol Sci

      Volume: 62 Issue: 3 Pages: 169-175

    • DOI

      10.1016/j.jdermsci.2011.03.003

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 喉頭粘膜病変を主症状とした抗ラミニン332型粘膜類天疱瘡の1例2013

    • Author(s)
      市村知佳、陳怡如、高田裕子、石井健、峯村佐和子、石井文人、橋本隆、石河晃
    • Organizer
      第35会水疱症研究会
    • Place of Presentation
      大分
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨髄異形成症候群に合併した抗p200類天疱瘡の1例2012

    • Author(s)
      小原芙美子,橋本由起,漆畑真理,鷲崎久美子,石井健,関東裕美,石河晃
    • Organizer
      第34回水疱症研究会
    • Place of Presentation
      青森
    • Year and Date
      2012-10-06
    • Related Report
      2013 Final Research Report
  • [Presentation] Analysis of epitope profile of autoantibodies in pemphigus vulgaris using domain-swapped Dsg3/Dsg2 ELISAs2012

    • Author(s)
      Saleh M, Ken Ishii, Jun Yamagami, Takahisa Hachiya, Masayuki Amagai
    • Organizer
      42nd Annual European Society of Dermatology Research Meeting
    • Place of Presentation
      Venice, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] What's new in bullous disease? What's new session2011

    • Author(s)
      Ishii K
    • Organizer
      22nd World Congress of Dermatology
    • Place of Presentation
      Seoul Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] Human epidermal tight junctions are functional and allow penetration of activated Langerhans cell dendrites2011

    • Author(s)
      Yoshida K, Kubo A, Yokouchi M, Ishii K, Kawasaki H, Ebihara T, Ohyama M, Nagao K, Amagai M
    • Organizer
      71th Annual meeting for The Society For Investigative Dermatology
    • Place of Presentation
      Phoenix
    • Related Report
      2013 Final Research Report
  • [Presentation] What’s new in bullous disease?2011

    • Author(s)
      Ishii K
    • Organizer
      22nd World Congress of Dermatology(招待講演)
    • Place of Presentation
      韓国、ソウル
    • Related Report
      2011 Research-status Report
  • [Presentation] Analysis of epitope profile of autoantibodies in pemphigus vulgaris using domain-swapped Dsg3/Dsg2 ELISAs

    • Author(s)
      Saleh M, Ken Ishii, Jun Yamagami, Takahisa Hachiya, Masayuki Amagai
    • Organizer
      42nd Annual European Society of Dermatology Research Meeting
    • Place of Presentation
      イタリア、ベニス
    • Related Report
      2012 Research-status Report
  • [Book] In vitro pathogenicity assay for anti-desmoglein autoantibodies in pemphigus. Molecular Dermatology Methods and Protocols Methods Mol Biol. Vol. 9612013

    • Author(s)
      Ishii K, Amagai M
    • Publisher
      Springer Science
    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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