| Project/Area Number |
23591630
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TERUI Tadashi 日本大学, 医学部, 教授 (30172109)
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| Project Period (FY) |
2011 – 2013
|
| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 皮膚科学 / 皮膚腫瘍学 / 癌 / 遺伝学 / ゲノム / 悪性黒色腫 / メチル化 / エピジェネティクス / DNAメチル化 / 早期鑑別診断 / 色素性母斑 |
|---|
| Research Abstract |
In order to identify the early tumor marker of malignant melanoma, Sequenom MassARRAY quantitative methylation analysis using the MassARRAY Compact System was performed for the quantitative DNA methylation analysis of ZAR1 gene. Fifth surgical specimens (e.g. melanocytic nevus, Spitz nevus, dysplastic nevus, and melanoma in situ) that were similar to malignant melanoma by pathologic diagnosis were examined. DNA were collected by using Leica Laser Microdissection systems. However, the extraction of DNA and the quantitative DNA methylation analysis of ZAR1 gene using the MassARRAY were difficult, therefore we were not able to analyze.
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