Analysis of unknown autoantigen for autoimmune bullous disease.

• Project/Area Number: 23591634
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Kurume University
• Principal Investigator: ARAKAWA Masataka 久留米大学, 医学部, 助教 (70441623)
• Co-Investigator(Kenkyū-buntansha): TSUCHISAKA Atsunari 久留米大学, 医学部, 研究員 (50599313) ; HASHIMOTO Takashi 久留米大学, 医学部, 教授 (20129597)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 皮膚診断学 / 皮膚免疫学 / 自己免疫性水疱症 / 薬剤誘発性天疱瘡 / 粘膜類天疱瘡 / 腫瘍随伴性天疱瘡

Research Abstract

We reported characteristics of 17 drug-induced pemphigus (DIP) patients for their clinical manifestations, causative drugs and the results of ELISA and immunoblotting (IB). This study indicated that the majority of the DIP cases studied showed pemphigus foliaceus-type phenotype with anti-Dsg1 autoantibodies, caused by thiol-containing drugs. We determined autoantigen for large number of pure ocular mucous membrane pemphigoid (pure OMMP) patients by IB using newly established hemidesmosome-rich fraction. We suggested that, although antigens for pure OMMP are heterogeneous, 4 integrin is the major and possibly most important autoantigen.
We tested a large number of paraneoplastic pemphigus (PNP) sera by various methods to assess how frequently anti- A2ML1 autoantibodies are detected in PNP. Our results demonstrated that autoantibodies to A2ML1 are frequently and specifically detected and may play a pathogenic role in PNP.