| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Research Abstract |
Bullous pemphigoid (BP) is an autoimmune blistering disease caused by IgG autoantibodies against the NC16a domain of BP180. In this study, we attempted to isolate IgG monoclonal antibodies against NC16a and identify epitopes strongly associated with the pathogenesis of BP. Antigen-specific antibody-secreting cells (ASCs) were collected from the patient's peripheral blood monocytes using an Immunospot array assay on a chip (ISAAC). IgG variable region cDNA was amplified from each ASC and ligated to an expression vector. Monoclonal antibodies were obtained from the culture supernatant of HEK293T cells transfected with the expression vectors. These monoclonal antibodies specifically recognized 542-566 amino acids of BP180. We perform to examine whether the antibodies induce blister formation in the human skin and to obtain other types of monoclonal antibodies from BP patients. In addition, we plan to develop a new therapy targeting disease-related antibodies.
|
