The development of the method of producing cultured skin using adipose tissue-derived mesenchymal stem cells.

• Project/Area Number: 23591647
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: KENJI Kameda 愛媛大学, 総合科学研究支援センター, 講師 (60363264)
• Co-Investigator(Kenkyū-buntansha): SHIRAKATA Yuji 愛媛大学, 大学院医学系研究科, 准教授 (50226320)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 再生医療 / 三次元培養 / 脂肪細胞 / 幹細胞 / 角化細胞 / 脂肪組織 / 表皮角化細胞

Outline of Final Research Achievements

We cultured adiposed-derived mesenchymal stem cells (ASCs), but cytokeratin-positive cells could not be detected. Therefore we seeded and gelled ASCs with Type I collagen solutions on the culture inserts, and then added the appropriate amount of 10 % FCS/DMEM inside and outside the inserts. And then were plated onto the gel cultures were stratified perform air exposure. We demonstrated three-dimensional configuration of the skin with HE staining. Three-dimensional reconstruction of the skin was subjected to HE staining was confirmed. It shows that ASCs can function as an alternative to dermal fibroblasts.