|Budget Amount *help
¥5,070,000 (Direct Cost : ¥3,900,000、Indirect Cost : ¥1,170,000)
Fiscal Year 2014 : ¥1,170,000 (Direct Cost : ¥900,000、Indirect Cost : ¥270,000)
Fiscal Year 2013 : ¥1,170,000 (Direct Cost : ¥900,000、Indirect Cost : ¥270,000)
Fiscal Year 2012 : ¥1,300,000 (Direct Cost : ¥1,000,000、Indirect Cost : ¥300,000)
Fiscal Year 2011 : ¥1,430,000 (Direct Cost : ¥1,100,000、Indirect Cost : ¥330,000)
|Outline of Final Research Achievements
We cultured adiposed-derived mesenchymal stem cells (ASCs), but cytokeratin-positive cells could not be detected. Therefore we seeded and gelled ASCs with Type I collagen solutions on the culture inserts, and then added the appropriate amount of 10 % FCS/DMEM inside and outside the inserts. And then were plated onto the gel cultures were stratified perform air exposure. We demonstrated three-dimensional configuration of the skin with HE staining. Three-dimensional reconstruction of the skin was subjected to HE staining was confirmed. It shows that ASCs can function as an alternative to dermal fibroblasts.