• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of IL-33 and its reseptor in contact hypersensitivity reaction

Research Project

Project/Area Number 23591652
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dermatology
Research InstitutionJichi Medical University

Principal Investigator

KOMINE Mayumi  自治医科大学, 医学部, 准教授 (00282632)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsIL-33 / ケラチノサイト / 発現調節 / インターフェロンγ / UVB / 表皮細胞 / IL-33 / 炎症性皮膚疾患 / IFNγ / IL-17 / ケモカイン / シグナル伝達
Research Abstract

We investigated the hypersensitivity reaction to FITC and oxazolone in wild type and soluble ST2 transgenic mice. There were no statistical differences in ear thickness and histopathological observasion in these mice. UVB, IFNg, and IL-17 induced IL-33 expression in protein and mRNA levels in cultured normal human epidermal keratinocytes (NHEKs). The induction of IL-33 expression in NHEKs was dependent on EGFR and ERK with these stimulations. In case of IL-17 and IFNg, the induction was dependent also on STAT1 and JAK. We have identified three distinct exon1s in the upstream of exon2. The putative promoter regions of IL-33 were cloned into luciferase vectors, and we identified active promoter region in the 5'flanking region of second exon1 (exon1b). This promoter region was active in NHEKs, normal human dermal fibroblasts, and human umbilical vein endothelial cells. The promoter activity was induced by the stimulation with IFNg and IL-17, consistent to our protein and mRNA results.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (37 results)

All 2013 2012 2011 2010

All Journal Article (20 results) (of which Peer Reviewed: 12 results) Presentation (17 results)

  • [Journal Article] Possible involvement of SDF-1/CXCL12 in the pathogenesis of Degos disease2013

    • Author(s)
      Meephansan J, Komine M, Hosoda S, Tsuda H, Karakawa M, Murata S, Demitsu T, Ohtsuki M
    • Journal Title

      J Am Acad Dermatol

      Volume: 68(1) Pages: 138-43

    • Related Report
      2013 Final Research Report
  • [Journal Article] Case of pemphigoid involving skin and mucous membrane with immunoglobulin G autoantibodies targeted to BP180 and laminin-332.2013

    • Author(s)
      Hosoda S, Suzuki M, Komine M, Fujita E, Yamada T, Murata S, Koga H, Hashimoto T, Ohtsuki M.
    • Journal Title

      J Dermatol

      Volume: 40 Issue: 12 Pages: 10671068-10671068

    • DOI

      10.1111/1346-8138.12311

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peritoneal loose body: A case report and comparison with encapsulated fat necrosis.2013

    • Author(s)
      Maekawa T, Komine M, Murata S, Ohtsuki M.
    • Journal Title

      J Dermatol

      Volume: 40 Issue: 12 Pages: 1058-1058

    • DOI

      10.1111/1346-8138.12336

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reagents inducing epidermal proliferation also induce pigmentation: Induction of keratinocyte proliferation as a novel strategy for the treatment of vitiligo2013

    • Author(s)
      Wu CS, Komine M, Fujimoto S, Ohmatsu H, Kikuchi K, Tada Y, Yu HS, Ohtsuki M, Tamaki K.
    • Journal Title

      J Dermatol Sci

      Volume: 72 Issue: 1 Pages: 66

    • DOI

      10.1016/j.jdermsci.2013.05.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of IL-33 in the epidermis: The mechanism of induction by IL-17.2013

    • Author(s)
      Meephansan J, Komine M, Tsuda H, Karakawa M, Tominaga S, Ohtsuki M.
    • Journal Title

      J Dermatol Sci

      Volume: 71 Issue: 2 Pages: 107114-107114

    • DOI

      10.1016/j.jdermsci.2013.04.014

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pustular eruption induced by sorafenib in a case of psoriasis vulgaris.2013

    • Author(s)
      Maki N, Komine M, Takatsuka Y, Maekawa T, Murata S, Ohtsuki M.
    • Journal Title

      J Dermatol

      Volume: 40 Issue: 4 Pages: 299-299

    • DOI

      10.1111/1346-8138.12097

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of IL-33 in the epidermis: The mechanism of induction by IL-172013

    • Author(s)
      Meephansan J, Komine M, Tsuda M, Karakawa M, Tominaga S, Ohtsuki M
    • Journal Title

      J Dermatol Sci

      Volume: 未定

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Suppressive effect of calcipotriol on the induction of matrix metalloproteinase (MMP)-9 and MMP-13 in a human squamous cell carcinoma cell line2012

    • Author(s)
      Meephansan J, Komine M, Tsuda H, Ohtsuki M
    • Journal Title

      Clin Exp Dermatol

      Volume: 37(8) Pages: 889-96

    • Related Report
      2013 Final Research Report
  • [Journal Article] Novel splice variants of IL-33: differential expression in normal and transformed cells2012

    • Author(s)
      Tsuda H, Komine M, Karakawa M, Etoh T, Tominaga S, Ohtsuki M
    • Journal Title

      J Invest Dermatol

      Volume: 132(11) Pages: 2661-4

    • Related Report
      2013 Final Research Report
  • [Journal Article] Regulation of IL-33 expression by IFN-γand tumor necrosis factor-αin normal human epidermal keratinocytes2012

    • Author(s)
      Meephansan J, Tsuda H, Komine M, Tominaga S, Ohtsuki M
    • Journal Title

      J Invest Dermatol

      Volume: 132(11) Pages: 2593-600

    • Related Report
      2013 Final Research Report
  • [Journal Article] The levels of soluble ST2 in sera and bullous fluid from patients with bullous pemphigoid2012

    • Author(s)
      Wakatabi K, Komine M, Meephansan J, Matsuyama Y, Tsuda H, Tominaga S, Ohtsuki M
    • Journal Title

      Eur J Dermatol

      Volume: 22(3) Pages: 333-6

    • Related Report
      2013 Final Research Report
  • [Journal Article] Ultraviolet B irradiation induces the expression of IL-33 mRNA and protein in normal human epidermal keratinocytes2012

    • Author(s)
      Meephansan J, Komine M, Tsuda H, Tominaga S, Ohtsuki M
    • Journal Title

      J Dermatol Sci

      Volume: 65(1) Pages: 72-4

    • Related Report
      2013 Final Research Report
  • [Journal Article] Sustained elevation of interleukin-33 in sera and synovial fluids from patients with rheumatoid arthritis non-responsive to anti-tumor necrosis factor : possible association with persistent IL-1βsignaling and a poor clinical response2012

    • Author(s)
      Matsuyama Y, Okazaki H, Hoshino M, Onishi S, Kamata Y, Nagatani K, Nagashima T, Iwamoto M, Yoshio T, Ohto-Ozaki H, Tamemoto H, Komine M, Sekiya H, Tominaga S, Minota S
    • Journal Title

      Rheumatol Int

      Volume: 32(5) Pages: 1397-401

    • Related Report
      2013 Final Research Report
  • [Journal Article] 表皮角化細胞におけるIL-33産生機序について2012

    • Author(s)
      ジトラダミーファンサン、小宮根真弓、津田英利、富永眞一、大槻マミ太郎
    • Journal Title

      医薬の門

    • Related Report
      2013 Final Research Report
  • [Journal Article] Regulation of IL-33 expression by IFN-γ and tumor necrosis factor-α in normal human epidermal keratinocytes.2012

    • Author(s)
      Meephansan, J., Tsuda, H., Komine, M., Tominaga, S., and Ohtsuki, M
    • Journal Title

      J. Invest. Dermatol

      Volume: 132 Issue: 11 Pages: 2593-2600

    • DOI

      10.1038/jid.2012.185

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel splice variants of IL-33: Differential expression in normal and transformed cells2012

    • Author(s)
      Tsuda, H., Komine, M., Karakawa, M., Etoh, T., Tominaga, S., and Ohtsuki, M
    • Journal Title

      J. Invest. Dermatol

      Volume: 132 Issue: 11 Pages: 2661-2664

    • DOI

      10.1038/jid.2012.180

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] The levels of soluble ST2 in sera and bullous fluid from patients with bullous pemphigoid.2012

    • Author(s)
      Wakatabi, K., Komine, M., Meephansan, J., Matsuyama, Y., Tsuda, H., Tominaga, S., and Ohtsuki, M.
    • Journal Title

      Eur. J. Dermatol

      Volume: 22 Issue: 3 Pages: 333-336

    • DOI

      10.1684/ejd.2012.1706

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultraviolet B irradiation induces the production of IL-33 in normal human epidermal keratinocytes2012

    • Author(s)
      Meephansan J, Komine M, Tsuda H, Tominaga S, Ohtsuki M.
    • Journal Title

      Journal of Dermatological Science

      Volume: 65 Pages: 71-74

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel splice variants of IL-33; differential expression in normal and transformed cells2012

    • Author(s)
      Tsuda H, Komine M, Karakawa M, Tominaga S, Ohtsuki M.
    • Journal Title

      Journal of Investigative Dermatology

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Regulation of IL-33 expression by interferon-γ and tumor necrosis factor-α in normal human epidermal keratinocytes2012

    • Author(s)
      Meephansan J, Tsuda H, Komine M, Etoh T, Tominaga S, Ohtsuki M.
    • Journal Title

      Journal of Investigative Dermatology

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] IL-33 as a nuclear protein to affect proliferation and differentiation of normal human epidermal keratinocytes2013

    • Author(s)
      Hidetoshi Tsuda, Mayumi Komine, Tomoyuki Ohshio, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      International Investigative Dermatology
    • Place of Presentation
      Edinburgh, Scotland
    • Related Report
      2013 Final Research Report
  • [Presentation] ケラチノサイトにおけるヒトIL-33プロモーター領域の解析2013

    • Author(s)
      津田英利・小宮根真弓・大塩智之・富永眞一・大槻マミ太郎
    • Organizer
      第36回日本分子生物学学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-33 affects proliferation and differentiation of normal human epidermal keratinocytes2013

    • Author(s)
      Tsuda H, Komine M, Oshio T, Tominaga S, Ohtsuki M
    • Organizer
      International Investigative Dermatology
    • Place of Presentation
      Edinburgh
    • Related Report
      2013 Annual Research Report
  • [Presentation] IL-33ノックダウンにより正常ヒト表皮角化細胞の細胞周期はG1/SからG2/M期へシフトする2013

    • Author(s)
      津田英利、小宮根真弓、大塩智之、富永眞一、大槻マミ太郎
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
  • [Presentation] IL-33 as a nuclear protein to affect proliferation and differentiation of normal human epidermal keratinocytes2013

    • Author(s)
      Hidetoshi Tsuda, Mayumi Komine, Tomoyuki Ohshio, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      International Investigative Dermatology
    • Place of Presentation
      Edinburgh, UK
    • Related Report
      2012 Research-status Report
  • [Presentation] Regulation of IL-33 by inflammatory cytokines in normal human epidermal keratinocytes2012

    • Author(s)
      Jitlada Meephansan, Hidetoshi Tsuda, Mayumi Komine, Satomi Hosoda, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      分子皮膚科フォーラム
    • Place of Presentation
      青森
    • Year and Date
      2012-04-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of IL-33 by inflammatory cytokines in normal human epidermal keratinocytes2012

    • Author(s)
      Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      東京皮膚フォーラム
    • Place of Presentation
      丸の内、東京
    • Year and Date
      2012-03-03
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-33 is produced by normal human keraitnocytes with environmental and inflammatory stimuli : a novel"alarmin"in the skin2012

    • Author(s)
      Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      Annual meeting of Society for Investigative Dermatology
    • Place of Presentation
      North Carolina, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-17A up regulates IL-33 expression in keratinocytes; negative feedback loop in psoriatic epidermis2012

    • Author(s)
      Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      Annual meeting of European Society for Dermatological Research
    • Place of Presentation
      Venice, Italy
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of IL-33 expression by inflammatory cytokines and its putative role in inflammatory skin disorders2012

    • Author(s)
      Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      Annual Meeting of Japanese Society for Investigative Dermatology
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] Retinoids regulates the production of matrix metalloproteinases in the human epidermal keratinocyte cell line HaCaT2012

    • Author(s)
      Satomi Hosoda, Mayumi Komine, Masaru Karakawa, Jitlada Meephansanm, Hidetoshi Tsuda, Mamitaro Ohtsuki
    • Organizer
      Annual Meeting of Japanese Society for Investigative Dermatology
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel splice variants of IL-33 expressed in normal human keratinocyte2012

    • Author(s)
      Hidetoshi Tsuda, Mayumi Komine, Tomoyuki Oshio, Satomi Hosoda, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      Annual Meeting of Japanese Society for Investigative Dermatology
    • Place of Presentation
      沖縄
    • Related Report
      2013 Final Research Report
  • [Presentation] Regulation of IL-33 expression by inflammatory cytokines and its putative role in inflammatory skin disorders2012

    • Author(s)
      Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Tomoyuki Oshio, Masaru Karakawa, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      Japanese Society for Investigative Dermatology
    • Place of Presentation
      Okinawa
    • Related Report
      2012 Research-status Report
  • [Presentation] IL-33 is produced by normal human keraitnocytes with environmental and inflammatory stimuli: a novel “alarmin” in the skin.2012

    • Author(s)
      Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Shoichiro Yano, Shin-ichi Tominaga, Mamitaro Ohtsuki
    • Organizer
      Society for Investigative Dermatology
    • Place of Presentation
      Raleigh, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] IL-33は核内でケラチノサイトのproliferationに関与している2011

    • Author(s)
      津田英利・小宮根真弓・大塩智之・富永眞一・大槻マミ太郎
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2013 Final Research Report
  • [Presentation] IL-33 is produced by normal human keraitnocytes with environmental and inflammatory stimuli: a novel "alarmin" in the skin2011

    • Author(s)
      Meephansan J, Komine M, Tsuda H, Yano S, Tominaga S, Ohtsuki M.
    • Organizer
      74th Society for Investigative Dermatology
    • Place of Presentation
      USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Calcipotriol reduced induction of MMP-9 and MMP-13 in human squamous cell carcinoma cell line2010

    • Author(s)
      Meephansan J, Komine M, Ohtsuki M.
    • Organizer
      40th European Society for Dermatologial Research
    • Place of Presentation
      Helsinki
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi