Cutaneous immunoregulatory functions of a novel antimicrobial agent, catestatin

• Project/Area Number: 23591655
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Juntendo University
• Principal Investigator: NIYONSABA FRANCOIS 順天堂大学, 医学(系)研究科(研究院), 准教授 (60365640)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 抗菌ペプチド / 皮膚免疫 / 自然免疫 / ケラチノサイト / 好中球 / 抗菌物質 / マスト細胞

Research Abstract

Catestatin, initially believed to act as an neuroendocrine regulator, is an antimicrobial agent found in the skin keratinocytes and fibroblasts, and is over-expressed during wound healing and infection. In this study, we observed that Catestatin and the newly discovered antimicrobial peptides known as IDRs stimulated various functions of human keratinocytes and neutrophils, leading to the wound healing acceleration. Thus, in addition to their microbiocidal properties, skin-derived antimicrobial agents might also participate to the regulation of cutaneous host defense, inflammation and innate immune system through activation of keratinocytes.

Research Products

• [Journal Article] Human antimicrobial peptide LL-37 modulates proinflammatory responses induced by cytokine milieus and double-stranded RNA in human keratinocytes (2013)
  • Author(s): Xue Chen
  • Journal Title: Biochem Biophys Res Commun Volume: 433 Issue: 4 Pages: 532-537
  • DOI: 10.1016/j.bbrc.2013.03.024
  • Peer Reviewed
• [Journal Article] The innate defense regulator peptides IDR-HH2, IDR-1002, and IDR-1018 modulate human neutrophil functions. (2013)
  • Author(s): Niyonsaba F, Madera L, Afacan N, Okumura K, Ogawa H, Hancock RE.
  • Journal Title: J Leukoc Biol Volume: 94 Issue: 1 Pages: 159-70
  • DOI: 10.1189/jlb.1012497
  • Peer Reviewed
• [Journal Article] Modulation of neutrophil apoptosis by antimicrobial peptides. (2012)
  • Author(s): Isao Nagaoka
  • Journal Title: ISRN Microbiology Volume: 2012 Pages: 12-12
  • DOI: 10.5402/2012/345791
  • Peer Reviewed
• [Journal Article] Interleukin-36 cytokines enhance the production of host defense peptides psoriasin and LL-37 by human keratinocytes through activation of MAPKs and NF-κB (2012)
  • Author(s): Nguyen TT
  • Journal Title: J Dermatol Sci Volume: 68 Issue: 1 Pages: 63-6
  • DOI: 10.1016/j.jdermsci.2012.07.010
  • Peer Reviewed
• [Journal Article] Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes (2011)
  • Author(s): Hoq Md Imranul
  • Journal Title: JOURNAL OF DERMATOLOGICAL SCIENCE Volume: 64 Issue: 2 Pages: 108-118
  • DOI: 10.1016/j.jdermsci.2011.08.001
  • Peer Reviewed
• [Journal Article] Human catestatin enhances migration and proliferation of normal human epidermal keratinocytes (2011)
  • Author(s): Hoq MI, Niyonsaba F, Ushio H, Aung G, Okumura K, Ogawa H
  • Journal Title: Journal for Dermatological Science Volume: 64 (2) Pages: 108-118
  • Peer Reviewed
• [Journal Article] A neuroendocrine antimicrobial peptide, catestatin, stimulates interleukin-8 production from human keratinocytes via activation of mitogen-activated protein kinases (2011)
  • Author(s): Aung G, Niyonsaba F, Ushio H, Hoq MI, Ikeda S, Ogawa H, Okumura K.
  • Journal Title: Journal for Dermatological Science Volume: 61(2) Pages: 142-144
  • Peer Reviewed
• [Presentation] Synthetic immunomodulatory peptides IDR-HH2, IDR-1002 and IDR-1018 regulate neutrophil functions (2013)
  • Author(s): Niyonsaba F, Madera L, Okumura K, Ogawa H, Hancock REW
  • Organizer: 15^<th> International Congress of Immunology
  • Place of Presentation: ミラノ, イタリア
  • Year and Date: 2013-08-25
• [Presentation] Synthetic immunomodulatory peptides IDR-HH2, IDR-1002 and IDR-1018 regulate neutrophil functions (2013)
  • Author(s): Niyonsaba F et al.
  • Organizer: 15th International Congress of Immunology
  • Place of Presentation: イタリア、ミラノ
• [Presentation] Effects of human β-defensins on interleukin-37 production by human keratinocytes (2013)
  • Author(s): SmithritheeR, Niyonsaba F et al.
  • Organizer: 2013 International Investigative Dermatology
  • Place of Presentation: スコットランド、エジンバラ
• [Presentation] Human b-defensin-3 improves the epithelial tight junction (TJ) barrier through activation of glycogen synthase kinase-3 (GSK-3) and protein kinase C (PKC) (2013)
  • Author(s): Kiatsurayanon C, Niyonsaba F et al.
  • Organizer: 2013 International Investigative Dermatology
  • Place of Presentation: スコットランド、エジンバラ
• [Presentation] IL-36 cytokines enhance the production of host defense peptides LL-37 and psoriasin by human keratinocytes through activation of MAPKs and NF-kB (2013)
  • Author(s): Akiyama T, Nguyen TT, Niyonsaba F
  • Organizer: 2013 International Investigative Dermatology
  • Place of Presentation: スコットランド、エジンバラ
• [Presentation] Synthetic innate defense regulator peptides modulate various functions of human neutrophils (2012)
  • Author(s): Niyonsaba F, Madera L, Okumura K, Ogawa H, Hancock REW
  • Organizer: The 37^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: ロワジールホテル&スパタワー那覇, 沖縄
  • Year and Date: 2012-12-09
• [Presentation] Effects of IL-36 cytokines on S100A7/psoriasin and cathelicidin LL-37 expression and production by primary human keratinocytes (2012)
  • Author(s): Nguyen TT, Niyonsaba F, Akiyama T, Smithrithee R, Kiatsurayanon C, Ushio H, Ikeda S, Okumura K, Ogawa H
  • Organizer: The 37^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: ロワジールホテル&スパタワー那覇, 沖縄
  • Year and Date: 2012-12-08
• [Presentation] Innate defense regulator peptides modulate neutrophil functions (2012)
  • Author(s): Niyonsaba F, Madera L, Okumura K, Ogawa H, Hancock REW
  • Organizer: 第62日本アレルギー学会
  • Place of Presentation: 大阪国際会議場, 大阪
  • Year and Date: 2012-11-30
• [Presentation] Neuroendocrine antimicrobial peptide, catestatin, and its variants increase keratinocyte migration and proliferation (2011)
  • Author(s): Niyonsaba F, Hoq MI, Ushio H, Aung G, Okumura K, Ogawa H
  • Organizer: The 36^<th> Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 国立京都国際会館, 京都
  • Year and Date: 2011-12-09
• [Presentation] Human catestatin induces epidermal keratinocyte migration and proliferation (2011)
  • Author(s): Niyonsaba F, Hoq MI, Ushio H, Aung G, Okumura K, Ogawa H
  • Organizer: 第39日本免疫学会総会第
  • Place of Presentation: 幕張メッセ, 千葉
  • Year and Date: 2011-11-29
• [Presentation] ランチョンセミナー : ヒト抗菌物質の皮膚免疫調節機能に対する役割 (2011)
  • Author(s): ニヨンサバフランソワ
  • Organizer: 第58回ドキシンシンポジウム
  • Place of Presentation: 順天堂大学, 東京
  • Year and Date: 2011-07-07
• [Presentation] Human catestatin induces epidermal keratinocyte migration and proliferation (2011)
  • Author(s): Niyonsaba F
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 幕張メッセ（千葉）
• [Presentation] Neuroendocrine antimicrobial peptide, catestatin, and its variants increase epidermal keratinocyte migration and proliferation (2011)
  • Author(s): Niyonsaba F
  • Organizer: The 36th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 国立京都国際会館（京都）
• [Presentation] 教育講演：上皮細胞が産生する抗菌物質の免疫調節機能 (2011)
  • Author(s): ニヨンサバ フランソワ
  • Organizer: 第39回日本耳鼻咽喉科感染症研究会(招待講演)
  • Place of Presentation: ホテル東京ガーデンパレス
• [Presentation] ランチョンセミナー：ヒト抗菌物質の皮膚免疫調節機能に対する役割 (2011)
  • Author(s): ニヨンサバ フランソワ
  • Organizer: 第58回ドキシンシンポジウム(招待講演)
  • Place of Presentation: 順天堂大学 本郷キャンパス （東京）
• [Presentation] Synthetic innate defense regulator peptides modulate various functions of human neutrophils
  • Author(s): Niyonsaba F
  • Organizer: The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 沖縄
• [Presentation] Effects of IL-36 cytokines on S100A7/psoriasin and cathelicidin LL-37 expression and production by primary human keratinocytes
  • Author(s): Nguyen TT, Niyonsaba F et al.
  • Organizer: The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: 沖縄
• [Presentation] Novel roles of host defense peptides in cutaneous biology
  • Author(s): Niyonsaba F
  • Organizer: IEIIS2012 Homeostatic Inflammation Symposium
  • Place of Presentation: 東京
  • Invited