Development of mouse model for autoinflammatory disease, TRAPS (TNF-receptor associated periodic syndrome)
| Project/Area Number |
23591662
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 自己炎症性疾患 / ＴＲＡＰＳ / ＴＮＦ受容体 / マウス / TRAPS / TNF受容体 / TNF / TNFR |
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| Research Abstract |
To address the pathomechanism of autoinflammatory disease, TRAPS (TNF receptor associated periodic syndrome), we tried to develop a mouse model of TRAPS by a forced expression of mutant TNF receptor. cDNA with a mutation in TNF receptor gene was ligated into the expression plasmid vector under the CMV promoter. This plasmid was linearized and cut with restriction enzymes and injected into C57BL/6J mice fertilized eggs. Out of 99 mouse specimens analyzed with PCR (polymerase chain reaction), 3 specimens were found to contain inserted plasmid DNA. One mouse died in utero, one mouse died within a few weeks after the birth and one mouse passed away at the age of 6 weeks after birth. The transgenic mouse at 6 weeks after the birth showed retardation in growth and abnormalities in hair follicle development and/or hair cycling. Taken together, these results suggested an important role of normal TNF receptor for development of hair follicles and other unspecified organs.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] A group of atopic dermatitis without IgE elevation or barrier impairment shows a high Th1 frequency: Possible immunological state of the intrinsic type2012
Author(s)
Kabashima-Kubo R, Nakamura M, Sakabe, J, Sigita K, Hino R, Mori T, Kobayashi M, Bito T, Kabashima K, Ogasawara K, Nomura Y, Nomura T, Akiyama M, Shimizu H, Tokura Y.
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Journal Title
J Dermatol Sci
Volume: 67
Issue: 1
Pages: 37-43
DOI
Related Report
Peer Reviewed
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