Combination of indocyanine green and gold nano-particles for diagnosis and treatment of tumors and vascular abnormality

• Project/Area Number: 23591663
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: National Defense Medical College
• Principal Investigator: AZUMA Ryuichi 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, 病院, 講師 (00531112)
• Co-Investigator(Kenkyū-buntansha): KIYOSAWA Tomoharu 防衛医科大学校病院, 准教授 (90221217) ; MORIMOTO Yuji 防衛医科大学校, 医学科専門課程, 准教授 (10449069)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: インドシアニングリーン / 金ナノロッド / 近赤外レーザー / EPR効果 / 血管腫

Outline of Final Research Achievements

Infrared laser irradiation after intravenous administration of indocyanine green increases vascular permeability at skin and dermo-muscular layer of rats. Subsequently induced intravenous gold nano-rods and infrared laser irradiation effectively injure skin and subcutaneous tissue. This indicates that GNRs would be highly concentrated in the tissue by EPR effect. The effect exerts intensely at superficial layer of the skin and is unaffected by blood flow. Thus, this method could be available for drug delivery system on tissues which have poor blood flow.

Research Products

• [Journal Article] Effective wound healing in streptozotocin-induced diabetic rats by adipose-derived stromal cell transplantation in plasma-gel containing fragmin/protamine microparticles. (2015)
  • Author(s): Sumi Yuki, Azuma Ryuichi et al
  • Journal Title: annals of plastic surgery Volume: 72 Pages: 113-120
  • Peer Reviewed / Open Access
• [Journal Article] Effective Wound Healing in Streptozotocin-Induced Diabetic Rats by Adipose-Derived Stromal Cell Transplantation in Plasma-Gel Containing Fragmin/Protamine Microparticles (2013)
  • Author(s): 東隆一
  • Journal Title: Annals of plastic surgery Volume: 72 Pages: 113-120
  • Peer Reviewed
• [Journal Article] Transplantation of inbred adipose-derived stromal cells in rats with plasma gel containing fragmin/protamine microparticles and FGF-2 (2013)
  • Author(s): 東隆一
  • Journal Title: Journal of Biomedical Materials Research Part B: Applied Biomaterials Volume: 101 Pages: 784-791
  • Peer Reviewed
• [Presentation] ICG蛍光造影法による腸管の血流評価 (2015)
  • Author(s): 東隆一 他
  • Organizer: 第58回日本形成外科学会
  • Place of Presentation: 京都
  • Year and Date: 2015-04-08 – 2015-04-10
• [Presentation] インドシアニングリーン血管造影法を用いた穿通枝皮弁のデザイン (2013)
  • Author(s): 東隆一
  • Organizer: 日本形成外科学会
  • Place of Presentation: 東京