Analyses of lipid network using inflammatory dermatoses model mice

• Project/Area Number: 23591665
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dermatology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: YAMAMOTO Kei 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (30304504)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脂質代謝 / 脂質メディエーター / 皮膚疾患 / 接触性皮膚炎 / ホスホリパーゼＡ２ / 炎症収束 / 皮膚癌 / 乾癬 / ホスホリパーゼA2 / アレルギー / 質量分析 / リピドミクス / アトピー性皮膚炎 / モデルマウス

Research Abstract

Epidermal lipids play important roles in skin barrier and pathophysiological function. Although perturbed lipid metabolism often leads to skin abnormality, molecular mechanisms underlying this process still remain incompletely understood. In this research, we show that secreted PLA2 group IID (PLA2G2D) is preferentially expressed in dendritic cells (DCs) and displays a pro-resolving function. In hapten-induced contact dermatitis, resolution of inflammation was compromised in skin and LNs of PLA2G2D-deficient mice, in which the immune balance was shifted toward a proinflammatory state over an anti-inflammatory state. PLA2G2D in the LNs contributed to mobilization of a pool of DHA that could serve as precursors for pro-resolving lipid mediators such as resolvin D1. Our results highlight PLA2G2D as a"resolving sPLA2" that ameliorates inflammation through mobilizing pro-resolving lipid mediators and points to a potential use of this enzyme for treatment of inflammatory disorders.

Research Products

• [Journal Article] Lymphoid tissue phospholipase A_2 group IID resolves contact hypersensitivity by driving anti-inflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K., Taketomi, Y., Sato, H., Shimo, K., Kobayashi, T., Ishikawa, Y., Ishii, T., Nakanishi, H., Ikeda, K., Taguchi, R., Kabashima, K., Arita, M., Arai, H., Lambeau, G., Bollinger, J.M., Hara, S., Gelb, M.H., and Murakami, M.
  • Journal Title: J. Exp. Med. Volume: 210 Pages: 1217-1234
  • Peer Reviewed
• [Journal Article] Mast cell maturation is driven via a group III phospholipase A_2-prostaglandin D_2-DP1 receptor paracrine axis (2013)
  • Author(s): Taketomi, Y., Ueno, N., Kojima, T., Satom H., Murase, R., Yamamoto, K., Tanaka, S., Sakanaka, M., Nakamura, Nishito, Y., Kawana, M., Kambe, N., Ikeda, K., Taguchi, R., Nakamizo, S., Kabashima, K., Gelb, M.H., Arita, M., Yokomizo, T., Nakamura, M., Watanabe, K., Hirai, H., Nakamura, M., Okayama, Y., Ra, C., Aritake, K., Urade, Y., Morimoto, K., Sugimoto, Y., Shimizu, T., Narumiya, S., Hara, S., and Murakami, M.
  • Journal Title: Nat. Immunol. Volume: 14 Pages: 554-563
  • Peer Reviewed
• [Journal Article] 疾病における脂質代謝ネットワークの機能 (2013)
  • Author(s): 三木寿美、山本圭、村上誠
  • Journal Title: オレオサイエンス Volume: 13(10) Pages: 23-29
  • Peer Reviewed
• [Journal Article] Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving anti-inflammatory lipid mediators. (2013)
  • Author(s): Miki Y, Yamamoto K, Taketomi Y, Sato H, Shimo K, Kobayashi T, Ishikawa Y, Ishii T, Nakanishi H, Ikeda K, Taguchi R, Kabashima K, Arita M, Arai H, Lambeau G, Bollinger JM, Hara S, Gelb MH, Murakami M.
  • Journal Title: The Journal of Experimental Medicine Volume: 210 Pages: 1217-1234
  • DOI: 10.1084/jem.20121887
  • Peer Reviewed
• [Journal Article] Mast cell maturation is driven via a novel group III phospholipase A2–prostaglandin D2–DP1 receptor paracrine axis. (2013)
  • Author(s): Taketomi, Y., Morimoto, K., Murakami, M., et al., 他30名
  • Journal Title: Nat. Immunol. Volume: (印刷中) Pages: 544-563
  • DOI: 10.1038/ni.2586
  • Peer Reviewed
• [Journal Article] Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators (2013)
  • Author(s): Miki, Y., Yamamoto, K. et al
  • Journal Title: J. Exp. Med. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Group X secreted phospholipase A_2 limits the development of atheros clerosis in LDL receptor-null mice : GX sPLA_2 reduces atherosclerosis (2013)
  • Author(s): Ait-Oufella H, Herbin O, Lahoute C, Coatrieux C, Loyer X, Joffre J, Laurans L, Ramkhelawon B, Blanc-Brude O, Karabina S, Girard CA, Payré C, Yamamoto K, Binder CJ, Murakami M, Tedgui A, Lambeau G, Mallat Z.
  • Journal Title: Arterioscler. Thromb. Vase. Biol. Volume: 33 Pages: 466-473
  • DOI: 10.1161/atvbaha.112.300309
  • Peer Reviewed
• [Journal Article] Hair follicular expression and function of group X secreted phospholipase A2 in mouse skin. (2011)
  • Author(s): Yamamoto, K., Taketomi, T., Isogai, Y., Miki, Y., Sato, H., Masuda, S., Nishito, Y., Morioka, K., Ishimoto, Y., Suzuki, N., Yokoya, Y., Hanasaki, K., Ishikawa, Y., Ishii, T., Kobayashi, T., Fukami, K., Ikeda, K., Nakanishi, H., Taguchi, R., and Murakami, M.
  • Journal Title: J Biol Chem. Volume: 286 Pages: 11616-11631
  • DOI: 10.1074/jbc.m110.206714
  • Peer Reviewed
• [Journal Article] Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function. (2011)
  • Author(s): Sato, H., Isogai, Y., Masuda, S., Taketomi, Y., Miki, Y., Kamei, D., Hara, S., Kobayashi, T., Ishikawa, Y., Ishii, T., Ikeda, K., Taguchi, R., Ishimoto, Y., Suzuki, N., Yokota, Y., Hanasaki, K., Suzuki-Yamamoto, T., Yamamoto, K., and Murakami, M.
  • Journal Title: J Biol Chem Volume: 286 Pages: 11632-11648
  • DOI: 10.1074/jbc.m110.206755
  • Peer Reviewed
• [Journal Article] Analysis of two major intracellular phospholipase A2 (PLA2) in mast cells reveals crutial contribution of cytosolic PLA2α, not but Ca2+-independent PLA2β, to lipid mobilization in proximal mast cells and distal fibroblasts. (2011)
  • Author(s): Ueno, N., Taketomi, Y., Yamamoto, K., Hirabayashi, T., Kamei, D., Kita, Y., Shimizu, T., Shinzawa, K., Tsujimoto, Y., Ikeda, K., Taguchi, R., Murakami, M.
  • Journal Title: J Biol Chem. Volume: 186 Pages: 37249-37263
  • DOI: 10.1074/jbc.m111.290312
  • Peer Reviewed
• [Journal Article] Secreted phospholipase A2 revisited. (2011)
  • Author(s): Murakami, M., Taketomi, Y., Sato, H., and Yamamoto, K.
  • Journal Title: J Biochem. Volume: 150 Pages: 233-255
  • DOI: 10.1093/jb/mvr088
  • Peer Reviewed
• [Journal Article] Integrated lipidomics in the secreted phospholipase A2 biology. (2011)
  • Author(s): Murakami, M., et al
  • Journal Title: Int J Mol Sci Volume: 12 Pages: 1474-1495
  • DOI: 10.3390/ijms12031474
  • Peer Reviewed
• [Journal Article] Secreted phospholipase A2, lipoprotein hydrolysis, and atherosclerosis: Integration with lipidmics. (2011)
  • Author(s): Yamamoto, K., et al
  • Journal Title: Anal Bioanal Chem. Volume: 400 Pages: 1829-1842
  • DOI: 10.1007/s00216-011-4864-z
  • Peer Reviewed
• [Journal Article] Recent progress in phospholipase A2 research: From cells to animals to humans. (2011)
  • Author(s): Murakami, M., Taketomi, Y., Miki, Y., Sato, H., Hirabayashi, T., and Yamamoto, K.
  • Journal Title: Prog Lipid Res. Volume: 50 Pages: 152-192
  • DOI: 10.1016/j.plipres.2010.12.001
  • Peer Reviewed
• [Journal Article] ホスホリパーゼA2 (2011)
  • Author(s): 山本圭
  • Journal Title: 生化学 Volume: 83 Pages: 449-461
  • Peer Reviewed
• [Journal Article] The adipocyte-inducible secreted phospholipases PLA2G5 and PLA2G2E play distinct roles in obesity
  • Author(s): Sato, H., Taketomi, Y., Ushida, A., Isogai, Y., Kojima, T., Hirabayashi, T., Miki, Y., Yamamoto, K., Nishito, Y., Kobayashi, T., Ikeda, K., Taguchi, R., Hara, S., Ida, S., Miyamoto, Y., Watanabe, M., Baba, H., Miyata, K., Oike, Y., Gelb, M.H., and Murakami, M.
  • Journal Title: Cell Metabolism Volume: (in press) Pages: 2014-2014
  • Peer Reviewed
• [Presentation] Epidermal phospholipase A_2 group IIF regulates keratinocyte differentiation and skin barrier through driving a unique set of lipid products (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau, G, and Murakami, M.
  • Organizer: 16th GEM/10th GERLI meeting 2013 GEM/GERLI Lipidomics meeting
  • Place of Presentation: Nice, France
• [Presentation] Epidermal phospholipase A_2 group IIF regulates keratinocyte differentiation and skin barrier through driving a unique set of lipid products (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau, G, and Murakami, M.
  • Organizer: 13^<th> International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases
  • Place of Presentation: San Juan, Puerto Rico
• [Presentation] IIF型分泌性ホスホリパーゼA_2は皮膚疾患の進行に寄与する (2013)
  • Author(s): 山本圭、三木寿美、佐藤真利子、椛島健治、石川由起雄、石井壽晴、村上誠
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 横浜
• [Presentation] IIF型分泌性ホスホリパーゼA_2は表皮の恒常性維持および病態に寄与する (2013)
  • Author(s): 山本圭、三木寿美、佐藤真利子、武富芳隆、池田和貴、中西広樹、田口良、村上誠
  • Organizer: 第55回日本脂質生化学会
  • Place of Presentation: 宮城県松島町
• [Presentation] GROUP IIF PHOSPHOLIPASE A_2 MAINTAINS SKIN HOMEOSTASIS (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau, G. and Murakami, M.
  • Organizer: 5th International Conference on Phospholipase A_2 Mediates Signaling in Translational Medicine
  • Place of Presentation: New Orleans(米国)
• [Presentation] GROUP IIF PHOSPHOLIPASE A2 MAINTAINS SKIN HOMEOSTASIS. (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau, G. and Murakami, M.
  • Organizer: 5th International Conference on Phospholipase A2 Mediates Signaling in Translational Medicine
  • Place of Presentation: New Orleans, USA
• [Presentation] IIF型分泌性ホスホリパーゼA2は皮膚疾患の進行に寄与する (2013)
  • Author(s): 山本圭、三木寿美、佐藤真利子、椛島健治、石川由起雄、石井壽晴、村上誠
  • Organizer: 第86回日本生化学会
  • Place of Presentation: 神奈川県横浜市
• [Presentation] Epidermal phospholipase A2 group IIF regulates keratinocyte differentiation and skin barrier through driving a unique set of lipid products. (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau, G, and Murakami, M.
  • Organizer: 13th International Conference on Bioactive Lipids in Cancer, Inflammation and Related Diseases.
  • Place of Presentation: San Juan, Pueruto Rico
• [Presentation] Epidermal phospholipase A2 group IIF regulates keratinocyte differentiation and skin barrier through driving a unique set of lipid products. (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau, G, and Murakami, M
  • Organizer: 16th GEM/10th GERLI meeting 2013 GEM/GERLI Lipidomics meeting.
  • Place of Presentation: Nice, France
• [Presentation] GROUP IIF PHOSPHOLIPASE A2 MAINTAINS SKIN HOMEOSTASIS (2013)
  • Author(s): Yamamoto, K., Miki, Y., Sato, M., Taketomi, Y., Gelb, M.H., Lambeau G., and Murakami, M.
  • Organizer: 5th International Conference on Phospholipase A2 Mediated Signaling in Translational Medicine
  • Place of Presentation: New Orleans
• [Presentation] IＩF型分泌性ホスホリパーゼA2は表皮の恒常性維持および病態に寄与する (2013)
  • Author(s): 山本圭、三木寿美、佐藤真利子、武富芳隆、池田和貴、中西広樹、田口良、村上誠
  • Organizer: 第５５回日本脂質生化学会
  • Place of Presentation: 仙台
• [Presentation] 皮膚病態生理機能におけるIIE型分泌性ホスホリパーゼA2の役割 (2012)
  • Author(s): 山本圭、佐藤弘泰、佐藤真利子、三木寿美、武富芳隆、村上誠
  • Organizer: 第８５回日本生化学会
  • Place of Presentation: 福岡
• [Presentation] 皮膚病態生理機能におけるIIE型分泌性ホスホリパーゼA_2の役割 (2012)
  • Author(s): 山本圭、佐藤弘泰、佐藤真利子、三木寿美、武富芳隆、村上誠
  • Organizer: 第85回日本生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-15
• [Presentation] Secreted phospholipase A_2s in skin homeostasis (2011)
  • Author(s): Yamamoto, K., Shimo, K., Miki, Y., Sato, M., Taketomi, Y., Ikeda, K., Nakanishi, H., Taguchi, R., Gelb, M.H., Lambeau, G., and Murakami, M.
  • Organizer: New Aspect of Phospholipid Biology and Medicine 2011
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-15
• [Presentation] Hair follicular expression and function of group X secreted phospholipase A_2 in mouse skin (2011)
  • Author(s): Yamamoto, K., Taketomi, Y., Miki, Y., Sato, H., Ikeda, K., Nakanishi, H., Taguchi, R., and Murakami, M.
  • Organizer: The 8^<th> GERLI Lipidomics meeting
  • Place of Presentation: Lyon, France
  • Year and Date: 2011-10-27
• [Presentation] Group IIF phospholipase A_2 maintains epidermal homeostasis (2011)
  • Author(s): Yamamoto, K., Shimo, K., Miki, Y., Taketomi, Y., Ikeda, K., Nakanishi, H., Taguchi, R., Gelb, M.H., Lambeau, G., and Murakami, M.
  • Organizer: The 12^<th> International Conference on Bioactive Lipids in Inflammation, Cancer and Related Diseases
  • Place of Presentation: Seattle, USA
  • Year and Date: 2011-09-19
• [Presentation] 皮膚病態生理における分泌性ホスホリパーゼA2の機能
  • Author(s): 山本圭
  • Organizer: 第84回日本生化学会(招待講演)
  • Place of Presentation: 京都
• [Presentation] 外根鞘に局在するsPLA2-Xは体毛の恒常性維持に関与する
  • Author(s): 山本圭
  • Organizer: 第53回脂質生化学会
  • Place of Presentation: 東京
• [Presentation] 樹状細胞のsPLA2-IIDは炎症応答の寛解を制御する
  • Author(s): 三木寿美
  • Organizer: 第53回脂質生化学会
  • Place of Presentation: 東京
• [Presentation] 脂肪過栄養により誘導されるsPLA2-Vは全身的な脂質代謝異常を制御する
  • Author(s): 佐藤弘泰
  • Organizer: 第53回脂質生化学会
  • Place of Presentation: 東京
• [Presentation] Roles of multiple sPLA2 enzymes in obesity, hepatic steatosis, insulin tolerance, lipoprotein metabolism and adipose inflammation.
  • Author(s): Sato, H.
  • Organizer: The 30th Naito Conference on Membrane Dynamics and Lipid Biology
  • Place of Presentation: 札幌
• [Presentation] Metabolic pathways regulating formation and composition of lipid droplets in sebocytes.
  • Author(s): Hirabayashi, T.
  • Organizer: The 30th Naito Conference on Membrane Dynamics and Lipid Biology
  • Place of Presentation: 札幌
• [Presentation] Group IIF phospholipase A2 maintains epidermal homeostasis. The 12th International Conference on Bioactive Lipids in Inflammation, Cancer and Related Diseases.
  • Author(s): Yamamoto, K.
  • Organizer: The 12th International Conference on Bioactive Lipids in Inflammation, Cancer and Related Diseases.
  • Place of Presentation: Seattle, USA
• [Presentation] Biology of group X secreted phospholipase A2: from physiology to cancer and inflammatory diseases.
  • Author(s): Lambeau, G.
  • Organizer: The 12th International Conference on Bioactive Lipids in Inflammation, Cancer and Related Diseases.
  • Place of Presentation: Seattle, USA
• [Presentation] Getting new insights into the functions of sPLA2s.
  • Author(s): Murakami, M.
  • Organizer: The 12th International Conference on Bioactive Lipids in Inflammation, Cancer and Related Diseases.
  • Place of Presentation: Seattle, USA
• [Presentation] アトピー性皮膚炎モデルにおける分泌性ホスホリパーゼA2の機能解析.
  • Author(s): 下華奈子
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] X型分泌性ホスホリパーゼA2の消化、神経機能、体毛形成における新規機能
  • Author(s): 佐藤弘泰
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] X型分泌性ホスホリパーゼA2の生殖における新規機能.
  • Author(s): 牛田絢子
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] ホスファチジルエタノールアミンは破骨細胞融合に関与する.
  • Author(s): 入江敦
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
• [Presentation] sPLA2-IIDは抗炎症性脂質の産生を制御して炎症応答の寛解に関与する
  • Author(s): 三木寿美
  • Organizer: ファーマ・バイオフォーラム2011
  • Place of Presentation: 仙台
• [Presentation] Hair follicular expression and function of group X secreted phospholipase A2 in mouse skin.
  • Author(s): Yamamoto, K
  • Organizer: The 8th GERLI Lipidomics meeting
  • Place of Presentation: Lyon, France
• [Presentation] Secreted Phospholipase A2 in epidermal differentiation, acidification, and barrier function.
  • Author(s): Murakami, M.
  • Organizer: The 8th GERLI Lipidomics meeting
  • Place of Presentation: Lyon, France
• [Presentation] Secreted phospholipase A2s in skin homeostasis.
  • Author(s): Yamamoto, K.
  • Organizer: New Aspect of Phospholipid Biology and Medicine 2011.
  • Place of Presentation: 福岡
• [Presentation] Secreted phospholipase A2s in atopic dermatitis.
  • Author(s): Shimo, K.
  • Organizer: New Aspect of Phospholipid Biology and Medicine 2011.
  • Place of Presentation: 福岡
• [Presentation] Secreted phospholipase A2s in metabolic syndrome.
  • Author(s): Sato, H.
  • Organizer: New Aspect of Phospholipid Biology and Medicine 2011.
  • Place of Presentation: 福岡
• [Presentation] Secreted phospholipase A2s in anaphylaxis.
  • Author(s): Taketomi, Y.
  • Organizer: New Aspect of Phospholipid Biology and Medicine 2011.
  • Place of Presentation: 福岡
• [Presentation] Secreted phospholipase A2s in reproduction.
  • Author(s): Ushida, A.
  • Organizer: New Aspect of Phospholipid Biology and Medicine 2011.
  • Place of Presentation: 福岡
• [Presentation] sPLA2と炎症収束
  • Author(s): 三木寿美
  • Organizer: 新学術研究「脂質マシナリー」若手ワークショップ
  • Place of Presentation: 東京
• [Presentation] sPLA2とアトピー性皮膚炎
  • Author(s): 下華奈子
  • Organizer: 新学術研究「脂質マシナリー」若手ワークショップ
  • Place of Presentation: 東京
• [Presentation] sPLA2とメタボリックシンドローム
  • Author(s): 佐藤弘泰
  • Organizer: 新学術研究「脂質マシナリー」若手ワークショップ
  • Place of Presentation: 東京
• [Presentation] sPLA2とアナフィラキシー
  • Author(s): 武富芳隆
  • Organizer: 新学術研究「脂質マシナリー」若手ワークショップ
  • Place of Presentation: 東京
• [Book] 論文ができてしまう！疾患モデルマウス表現型解析指南 (2011)
  • Author(s): 佐藤弘泰
  • Publisher: 中山書店
• [Remarks]
  • URL: http://www.igakuken.or.jp/lipid
• [Remarks] 体に蓄えられた魚の油が病気を止める ～体に優しい脂質を動かし炎症にブレーキをかける酵素の発見～
  • URL: http://www.igakuken.or.jp/research/topics/2013/0524.html
• [Remarks] 体に優しい脂質を働かせ、炎症にブレーキをかける酵素の同定
  • URL: http://www.igakuken.or.jp/lipid/research9.html
• [Remarks] アレルギーを起こす新しい脂質代謝経路を解明 ～花粉症等アレルギー克服に向けての新しい創薬に期待～
  • URL: http://www.igakuken.or.jp/research/topics/2013/0430.html
• [Remarks] マスト細胞成熟経路の発見
  • URL: http://www.igakuken.or.jp/lipid/reseacch8.html
• [Remarks]
  • URL: http://www.igakuken.or.jp/lipid/
• [Patent(Industrial Property Rights)] PLA2G2Fを指標とした表皮角化細胞の過増殖性疾患に対する薬剤スクリーニング方法 (2013)
  • Inventor(s): 村上誠、山本圭
  • Industrial Property Rights Holder: 公益財団法人東京都医学総合研究所
  • Industrial Property Number: 2013-240871
  • Filing Date: 2013-11-21
  • Application Year: 2013
  • Overseas
• [Patent(Industrial Property Rights)] PLA2G2Fを指標とした抗皮膚癌薬のスクリーニング方法 (2013)
  • Inventor(s): 村上誠、山本圭
  • Industrial Property Rights Holder: 村上誠、山本圭
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-11-21
  • Application Year: 2013
• [Patent(Industrial Property Rights)] PLA2G2Fを指標とした抗皮膚癌薬のスクリーニング方法 (2012)
  • Inventor(s): 村上誠、山本圭
  • Industrial Property Rights Holder: 公益財団法人東京都医学総合研究所
  • Industrial Property Number: 2012-255316
  • Filing Date: 2012-11-21
  • Application Year: 2012
  • Overseas