Interstitial pathophysiology and angiogenesis and its imaging in myocardial ischemia and remodeling

• Project/Area Number: 23591756
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Kanazawa University
• Principal Investigator: TAKI Junichi 金沢大学, 大学病院, 講師 (10251927)
• Co-Investigator(Kenkyū-buntansha): SHIBA Kazuhiro 金沢大学, 学際科学実験センター, 教授 (40143929) ; OGAWA Kazuma 金沢大学, 薬学系, 准教授 (30347471)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 心筋虚血 / 心筋梗塞 / メチオニン / 血管新生 / リモデリング / マクロファージ / 心筋繊維芽細胞 / テネイシンC / 心筋線維芽細胞 / Galactro-RGD

Research Abstract

We investigated the serial changes and mechanisms of C-14-methionine uptake in a rat model of myocardial ischemia and reperfusion. Both C-14-methionine and Tl-201 uptake were reduced in the area at risk at 1 day after reperfusion. However, 3 days after reperfusion, an increased C-14-methionine uptake was observed corresponding to the area of still reduced Tl-201 uptake, and the C-14-Methionine uptake gradually declined until 28 days. The C-14-methionine uptake area at 3-7 days corresponded well to the macrophage infiltrations demonstrated by positive CD68 staining. Anti alpha-SMA staining appeared at 7 days, after which CD68 staining was gradually replaced by the SMA staining, suggesting that methionine uptake in the early phase after ischemia and reperfusion might reflect inflammatory activity. Methionine imaging may be useful for inflammatory imaging early after myocardial infarction.

Research Products

• [Journal Article] ^<14>C-Methionine Uptake as a Potential Marker of Inflammatory Process after Myocardial Ischemia and Reperfusion (2013)
  • Author(s): Taki, J., Wakabayashi, H.,Inaki, A., Imanaka-Yoshida, K., Hiroe, M., Ogawa, K., Morooka M., Kubota K., Shiba, K., Yoshida, T., Kinuya, S.
  • Journal Title: J Nucl Med Volume: 54
  • NAID: 120005253902
  • Peer Reviewed
• [Journal Article] Optimization of iterative reconstruction parameters with attenuation correction, scatter correction and resolution recovery in myocardial perfusion SPECT/CT. (2013)
  • Author(s): Koichi Okuda, Kenichi Nakajima, Masato Yamada, Hiroshi Wakabayashi, Hajima Ichikawa, Hiroyuki Arai, Shinro Matsuo, Junichi Taki, Mitsumasa Hashimoto, Seigo Kinuya.
  • Journal Title: Ann Nucl Med Volume: 28 Issue: 1 Pages: 60-68
  • DOI: 10.1007/s12149-013-0785-6
  • NAID: 120005368235
  • Peer Reviewed / Open Access
• [Journal Article] Development and evaluation of a novel (99m)tc-labeled annexin A5 for early detection of response to chemotherapy (2013)
  • Author(s): Ogawa, K. Ohtsuki, K. Shibata, T. Aoki, M. Nakayama, M. Kitamura, Y. Ono, M. Ueda, M. Doue, T. Onoguchi, M. Shiba, K. Odani, A.
  • Journal Title: PLoS One Volume: 8 Issue: 12 Pages: e81191-e81191
  • DOI: 10.1371/journal.pone.0081191
  • NAID: 120006986751
  • Peer Reviewed
• [Journal Article] 14C-Methionine Uptake as a Potential Marker of Inflammatory Process after Myocardial Ischemia and Reperfusion (2013)
  • Author(s): Taki J, Wakabayashi H, Inaki A, Imanaka-Yoshida K, Hiroe M, Ogawa K, Morooka Miyako, Kubota K, Shiba K, Yoshida T, Kinuya S
  • Journal Title: J Nucl Med Volume: 54 Issue: 3 Pages: 431-436
  • DOI: 10.2967/jnumed.112.112060
  • NAID: 120005253902
  • Peer Reviewed
• [Journal Article] Assessment of Doxorubicin Cardiac Toxicity Using Gated ^99mTc-Hexakis-2-Methoxyisobutylisonitrile Myocardial Single Photon Emission Computed Tomography (2012)
  • Author(s): Wakabayashi H, Taki J, Inaki A, Sumiya H, Tsuchiya H, Kinuya S
  • Journal Title: Circulation Journal Volume: 76 Issue: 5 Pages: 1190-1196
  • DOI: 10.1253/circj.CJ-11-1174
  • NAID: 10030132674
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed
• [Journal Article] Standardization of metaiodobenzylguanidine heart-to-mediastinum ratio using a calibration phantom : Effects of correction on normal databases and a multi-center study (2012)
  • Author(s): Nakajima K, Okuda K, et al
  • Journal Title: Eur J Nucl Med Mol Imaging Volume: 39 Issue: 1 Pages: 113-9
  • DOI: 10.1007/s00259-011-1963-1
  • Peer Reviewed
• [Journal Article] 心臓核医学の最先端：SPECTにおける定量解析の進歩 (2012)
  • Author(s): 滝 淳一
  • Journal Title: PET journal Volume: No17 Pages: 22-24
• [Journal Article] Editorial : Potential utility of 99mTc-annexin V imaging in ischemically damaged myocardium (2011)
  • Author(s): Taki J., Matsunari I., Wakabayashi H., Inaki A., Kinuya S.
  • Journal Title: Intv Cardiol Volume: 2 Pages: 765-767
  • Peer Reviewed
• [Journal Article] テネイシンCと分子イメージング (2011)
  • Author(s): 滝 淳一、若林大志、稲木杏吏、絹谷清剛
  • Journal Title: 呼吸と循環 Volume: 59 Pages: 1071-1076
• [Journal Article] Quantification of myocardial perfusion SPECT using freeware package (cardioBull) (2011)
  • Author(s): Okuda K, Nakajima K, et al
  • Journal Title: Ann Nucl Med Volume: 25 Issue: 8 Pages: 571-9
  • DOI: 10.1007/s12149-011-0504-0
  • NAID: 10030232773
  • Peer Reviewed
• [Journal Article] Cause of apical thinning on attenuation-corrected myocardial perfusion SPECT (2011)
  • Author(s): Okuda K, Nakajima K, et al
  • Journal Title: Nucl Med Commun Volume: 32 Issue: 11 Pages: 1033-9
  • DOI: 10.1097/mnm.0b013e32834b69e1
  • NAID: 120003518404
  • Peer Reviewed
• [Presentation] Tc-99m-Annexin V Uptake as a Potential Marker of Left Ventricular Remodeling after Myocardial Ischemia and Reperfusion (2014)
  • Author(s): Taki J, Wakabayashi H, Matsunari I.
  • Organizer: The 78th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 東京国際フォーラム（ 東京）
• [Presentation] Postconditioning facilitate myocardial inflammatory resolution after severe ischemia and reperfusion: Assessment by C-14 methionine autoradiography (2014)
  • Author(s): Taki J, Wakabayashi H, Inaki A, Imanaka-Yoshida K, Hiroe M, Ogawa K, Shiba K, Yoshida T, Kinuya S
  • Organizer: Society of Nuclear Medicine and Molecular Imaging, 61st Annual Meeting
  • Place of Presentation: St. Louis Convention Center (USA)
• [Presentation] C-14-Methionine Accumulation as a Potential Marker of Inflammatory Process after Myocardial Ischemia and Reperfusion (2013)
  • Author(s): Taki, J., Wakabayashi, H., Imanaka -Yoshida, K., Matsunari, I., Hiroe, M.
  • Organizer: The 77th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Yokohama
  • Year and Date: 2013-03-15
• [Presentation] Tc-99m-Annexin-V Uptake correlates with Left Ventricular Remodeling in Rats with Ischemia and Reperfusion (2013)
  • Author(s): Taki J, Wakabayashi H, Inaki A, Blankenberg FG, Tait JF, Matsunari I, Kinuya S
  • Organizer: International Conference of Nuclear Cardiology and Cardiac CT
  • Place of Presentation: The Berliner Congress Centre (Germany)
• [Presentation] Early prediction of myocardial remodeling by Tc-99m Annexin-V uptake in a rat model of myocardial ischemia and reperfusion (2013)
  • Author(s): Wakabayashi H, Taki J, Inaki A, Shiba K, Kinuya S
  • Organizer: Society of Nuclear Medicine and Molecular Imaging, 60th Annual Meeting
  • Place of Presentation: Vancouver Convention Centre (Canada)
• [Presentation] Correlation between Tc-99m Annexin-V uptake and left ventricular remodeling in a rat with myocardial ischemia and reperfusion (2013)
  • Author(s): TakiJ, Wakabayashi H, Inaki A, Blankenberg F, Tait JF, Matsunari I, Shiba K, Kinuya S
  • Organizer: Society of Nuclear Medicine and Molecular Imaging, 60th Annual Meeting
  • Place of Presentation: Vancouver Convention Centre (Canada)
• [Presentation] マルチモダリティ時代の心臓核医学の役割と展望 (2013)
  • Author(s): 滝 淳一
  • Organizer: 第20回静岡県循環器核医学研究会
  • Place of Presentation: 静岡第一ホテル（ 静岡）
  • Invited
• [Presentation] 心筋アポトーシスと虚血再環流後の左室リモデリングの関係：Tc-99m-annexin Vイメージングによる検討 (2013)
  • Author(s): 滝 淳一、若林大志、稲木杏吏、柴 和弘、絹谷清剛
  • Organizer: 第53回日本核医学学術総会
  • Place of Presentation: 福岡国際会議場（ 福岡）
• [Presentation] マルチモダリティ時代の心臓核医学の意義 (2013)
  • Author(s): 滝 淳一
  • Organizer: 心臓核医学講演会
  • Place of Presentation: 佐賀大学医学部（ 佐賀）
  • Invited
• [Presentation] 虚血心の分子イメージングと血流SPECT (2013)
  • Author(s): 滝 淳一
  • Organizer: 第４３回ライラックカンファランス
  • Place of Presentation: KKRホテル札幌（ 北海道）
  • Invited
• [Presentation] 核医学的手法による微小循環障害の評価 (2013)
  • Author(s): 滝 淳一
  • Organizer: Beyond Angiography Japan XVIII
  • Place of Presentation: ブリズベイホテル（ 神奈川）
  • Invited
• [Presentation] C-14-Methionine Accumulation as a Potential Marker of Inflammatory Process after Myocardial Ischemia and Reperfusion (2013)
  • Author(s): Taki J, Wakabayashi H, Imanaka-yoshida K, Matsunari I, Hiroe M
  • Organizer: The 77th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: パシフィコ横浜（ 神奈川）
• [Presentation] ^1 Postconditioning attenuates interstitial tissue remodeling in a rat model of severe ischemia and reperfusion : Assessment by I-125-anti tenascin-C antibody imaging (2012)
  • Author(s): Taki, J., Wakabayashi, H.,Inaki, A., Imanaka-Yoshida, K., Hiroe, M., Ogawa, K., Shiba, K., Kinuya, S.
  • Organizer: 59th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: Miami Beach Convention Center (USA)
  • Year and Date: 2012-06-12
• [Presentation] Postconditioning attenuates interstitial tissue remodeling in a rat model of severe ischemia and reperfusion: Assessment by I-125-anti tenascin-C antibody imaging (2012)
  • Author(s): Taki J, Wakabayashi H, Inaki A, Imanaka-Yoshida K, Hiroe A,Kazuma O, Shiba K, Kinuya S
  • Organizer: 59th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: Maiami Convention Center (USA)
• [Presentation] ラット心筋虚血再還流モデルにおけるTenascin Cメージング:Postconditioningにおける検討 (2012)
  • Author(s): 滝 淳一、若林大志、稲木杏吏、廣江道昭、今中恭子、小川数馬、柴 和弘、松成一朗、絹谷清剛
  • Organizer: 第52回日本核医学会総会
  • Place of Presentation: ロイトン札幌（ 北海道）
• [Presentation] 心電図同期C-11 Hydroxyephedrine PETの検討 (2012)
  • Author(s): 松成一朗、滝 淳一、中嶋憲一、絹谷清剛
  • Organizer: 第52回日本核医学会総会
  • Place of Presentation: ロイトン札幌（ 北海道）
• [Presentation] Postconditioning Attenuates Tenascin-C Expression in a Rat Model of Severe Ischemia and Reperfusion: Assessement by I-125-anti Tenascin-C Antibody Imaging (2012)
  • Author(s): Taki J, Wakabayashi H, Imanaka-Yoshida K, Hiroe M, Matsunari I.
  • Organizer: The 76th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: 福岡国際会議場 （ 福岡市）
• [Presentation] Spatial and temporal change of C-14-methionine uptake in a rat model of acute ischemia and reperfusion (2011)
  • Author(s): Taki, J., Wakabayashi, H.,Inaki, A., Ogawa, K., Shiba, K., Morooka, M., Kubota, K., Hiroe, M., Kinuya, S.
  • Organizer: 58th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: San Antonio Convention Center (USA)
  • Year and Date: 2011-06-06
• [Presentation] Methionine uptake in a rat model of acute ischemia and reperfusion: Assessment by C-14 methionine autoradiography (2011)
  • Author(s): Taki J, Wakabayashi H, Hiroe M, Imanaka-Yoshida K, Kubota K, Matsuo S, Nakajima K, Matsunari I
  • Organizer: The 75th Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: パシフィコ横浜 （ 横浜市）
• [Presentation] Role of BMIPP Imaging in Chronic IHD, ACS, and CKD - The Japanese Perspective (2011)
  • Author(s): Taki J
  • Organizer: 58th Annual Meeting of the Society of Nuclear Medicine(招待講演)
  • Place of Presentation: San Antonio Convention Center (San Antonio)
• [Presentation] Spatial and temporal change of C-14-methionine uptake in a rat model of acute ischemia and reperfusion (2011)
  • Author(s): Taki J, Wakabayashi H, Inaki A, Kazuma O, Shiba K, Morooka M, Kubota K, Hiroe M, Kinuya S
  • Organizer: 58th Annual Meeting of the Society of Nuclear Medicine
  • Place of Presentation: San Antonio Convention Center (San Antonio)
• [Presentation] ラット心筋虚血再還流モデルにおけるC-14-メチオニンイメージング (2011)
  • Author(s): 滝 淳一、若林大志、稲木杏吏、諸岡都、窪田和雄、廣江道昭、今中恭子、小川数馬、柴 和弘、絹谷清剛
  • Organizer: 第51回日本核医学会総会
  • Place of Presentation: つくば国際会議場（ つくば市）
• [Book] Imaging the autonomic function with PET-CT and SPECT-CT (2014)
  • Author(s): Matsunari I, Taki J
  • Publisher: Springer-Verlag
• [Book] Role of Fatty Acid Imaging with ^<123>I-β-p-^<123>I-Iodophenyle -Pentadecanoic Acid (^<123>I-BMIPP) in Ischemic Heart Diseases. Ischemic Heart Disease (2013)
  • Author(s): Taki, J., Matsunari, I., Wakabayashi, H., Inaki, A., Kinuya, S.
  • Publisher: INTECH
• [Book] Ischemic Heart Disaese (2013)
  • Author(s): Taki J, Matsunari I, Wakabayashi H, Inaki A, Kinuya S
  • Total Pages: 200
  • Publisher: INTECH
• [Book] Apoptosis Imaging in Diseased Myocardium. 12 Chapters in Nuclear Medicne (2011)
  • Author(s): Taki, J., Wakabayashi, H., Inaki, A., Matsunari, I., Kinuya, S.
  • Publisher: INTECH
• [Book] 12 Chapters on Nuclear Medicine (2011)
  • Author(s): Taki J, Wakabayashi H, Inaki A, Matsunari I, Kinuya S
  • Total Pages: 304
  • Publisher: Apoptosis Imaging in Diseased Myocardium