Analysis of cellular radiation-sensitivity by phosphoproteomics
Project/Area Number |
23591834
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Radiation science
|
Research Institution | The University of Tokyo |
Principal Investigator |
ENOMOTO ATSUSHI 東京大学, 医学(系)研究科(研究院), 助教 (20323602)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | リン酸化プロテオーム / STK38 / 放射線感受性 / 放射線増感 / チェックポイント制御 / プロテオーム解析 / 放射線細胞応答 / リン酸化 |
Research Abstract |
STK38 (Serine/threonine kinase 38), also known as NDR1 (nuclear Dbf2-related 1), is a serine/threonine protein kinase belonging to a subclass of the protein kinase A (PKA)/PKG/PKC-like (AGC) family. I have recently reported that STK38 is activated by oxidative stresses such as X-irradiation or H2O2, and that knockdown of STK38 enhanced cellular sensitivity to DNA damage. To further clarify a role of STK38 in DNA damage response, I tried to identify a substrate of STK38 using phosoproteome analysis. I have identified not less than 30 in vitro substrates, some of which are known to be implicated in DNA damage response such as DNA repair, cell cycle checkpoint arrest, and apoptosis. I have investigated the significance of the STK38-mediated phosphorylation of their substrates
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Anti-tumor activity of olaparib, a poly (ADP-ribose) polymerase(PARP) inhibitor, in cultured endometrial carcinoma cells2014
Author(s)
A. Miyasaka, K. Oda, Y. Ikeda, O.Wada-Hiraike, T. Kashiyama, A. Enomoto, N.Hosoya, T. Koso, T. Fukuda, K. Inaaba, K.Sone, Y. Uehara, R. Kurikawa, K. Nagasaka,Y.Matsumoto, T. Arimoto, S. Nakagawa, H.Kuramoto, K. Miyagawa, T. Yano, K. Kawana,Y. Osuga, T. Fujii
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Anti-tumor activity of olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, in cultured endometrial carcinoma cells.2014
Author(s)
A. Miyasaka, K. Oda, Y. Ikeda, O. Wada-Hiraike, T. Kashiyama, A. Enomoto, N. Hosoya, T. Koso, T. Fukuda, K. Inaaba, K. Sone, Y. Uehara, R. Kurikawa, K. Nagasaka, Y.Matsumoto, T. Arimoto, S. Nakagawa, H. Kuramoto, K. Miyagawa, T. Yano, K. Kawana, Y. Osuga, T. Fujii.
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Journal Title
BMC Cancer
Volume: -
Issue: 1
Pages: 179-179
DOI
Related Report
Peer Reviewed
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[Journal Article] Evaluation of zinc (II) cheletors for inhibiting p53-mediated apoptosis2013
Author(s)
A. Morita, S. Ariyasu, S, Ohya, I.Takahashi, B. Wang, K. Tanaka, T. Uchida,H. Okazaki, K. Hanaya, A. Enomoto, M. Nenoi,M. Ikekita, S. Aoki, Y. Hosoi
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Journal Title
Oncotarget
Volume: 4
Pages: 2439-2449
Related Report
Peer Reviewed
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[Journal Article] Evaluation of zinc (II) cheletors for inhibiting p53-mediated apoptosis.2013
Author(s)
A. Morita, S. Ariyasu, S, Ohya, I. Takahashi, B. Wang, K. Tanaka, T. Uchida, H. Okazaki, K. Hanaya, A. Enomoto, M. Nenoi, M. Ikekita, S. Aoki, Y. Hosoi.
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Journal Title
Oncotarget
Volume: 4
Pages: 2439-2449
Related Report
Peer Reviewed
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