Misrepair using radiosensitizer and radiosensitivity under non cycling cells

• Project/Area Number: 23591849
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: Keio University
• Principal Investigator: KAWATA Tetsuya 慶應義塾大学, 医学部, 講師 (60234077)
• Co-Investigator(Kenkyū-buntansha): FUKADA Junichi 慶應義塾大学, 医学部, 講師 (50338159)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 幹細胞 / 静止期細胞 / 放射線増感 / ＰＬＤＲ / 誤修復 / 癌幹細胞 / 重粒子 / PLDR / 腫瘍幹細胞 / ATM / NBS1

Research Abstract

We have demonstrated whether AKT is important molecular targets to control the radioresistance through the activation of phospholitated AKT compared between CD133+ and CD133- glioma tumour cells after ionizing radiation treatment. We showed IR-induced AKT phosphorylation in CD133+ tumour cells than CD133- tumour cells when mirin was used. Our data suggested that the tumour cells expressing CD133+, a maker for the stem cell, was induced the activation of AKT more than CD133- cells after radiation. From this result, it may be said that CD133+cells have greater repair activation in response to DNA damage in cellular radiosensitivity. Confluent normal cells were exposed to X-rays and heavy ions and PLDR was studied using FISH technique. It was found that heavy ions induce similar exchanges between PLDR and PLD condition, suggesting that heavy ion therapy is as effective to non-cycling cells as cycling cells.

Research Products

• [Journal Article] Effects of shielding on the induction of 53BP1 foci and micronuclei after Fe ion exposures (2014)
  • Author(s): Hu W, Pei H, Li H, Ding N, He J, Wang J, Furusawa Y, Hirayama R, Matsumoto Y, Liu C, Li Y, Kawata T, Zhou G.
  • Journal Title: J Radiat Res Volume: 55(1) Issue: 1 Pages: 10-16
  • DOI: 10.1093/jrr/rrt078
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from the kinetics and fidelity of chromosome rejoining in normal human fibroblasts. (2013)
  • Author(s): Liu C, Kawata T, Zhou G, et al
  • Journal Title: J Radiat Res Volume: 54 Issue: 6 Pages: 989-997
  • DOI: 10.1093/jrr/rrt031
  • Peer Reviewed
• [Journal Article] Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET) (2013)
  • Author(s): Liu C, Kawata T, Furusawa Y, Zhou G, Inoue K, Fukada J, Kota R, George K, Cucinotta F, Okayasu R.
  • Journal Title: Mutat Res Volume: 756(1-2) Pages: 101-107
  • Peer Reviewed
• [Journal Article] Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET). (2013)
  • Author(s): Liu C, Kawata T, et al
  • Journal Title: Mutat Res Volume: 756 Issue: 1-2 Pages: 101-107
  • DOI: 10.1016/j.mrgentox.2013.05.005
  • Peer Reviewed
• [Journal Article] Comparison of the repair of potentially lethal damage after low- and high-LET radiation exposure, assessed from kinetics and fidelity of chromosome rejoining in normal human fibroblasts (2013)
  • Author(s): C LIU, T KAWATA, G ZHOU, Y FURUSAWA, R KOTA, A KUMABE, S SUTANI, J FUKADA, M MISHIMA, N SHIGEMATSU, K GEORGE and F CUCINOTTA
  • Journal Title: Journal of Radiation Research Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Chromosome aberrations in normal human fibroblasts analyzed in G0/G1 and G2/M phases after exposure in G0 to radiation with different linear energy transfer (LET) (2013)
  • Author(s): Cuihua LIU, Tetsuya KAWATA*, Yoshiya FURUSAWA, Guangming ZHOU, Kohei INOUE, Junichi FUKADA, Ryuichi KOTA, Kerry George, Francis Cucinotta, Ryuichi OKAYASU
  • Journal Title: Mutation Research Volume: 印刷中
  • Peer Reviewed
• [Presentation] Comparison of chromosome aberrations analyzed in G0/G1 and G2/M normal human fibroblasts (2013)
  • Author(s): Kawata t et al.
  • Organizer: 59th Radiation Research Society Annual Meeging
  • Place of Presentation: New Orleans USA
• [Presentation] Potentially lethal damage repair after low or high LET irradiation (2012)
  • Author(s): Kawata T, et al
  • Organizer: 58th Annual meeting of Radiation Research
  • Place of Presentation: サンワンプエルトリコ
• [Presentation] Hyper-radiosensitivity of Ataxia Telangiectasia exposed to radiation (2012)
  • Author(s): Tetsuya Kawata, et al
  • Organizer: The second international conference of Pekin University on radiation oncology
  • Place of Presentation: 北京大学中国
• [Presentation] Chromosomal aberrations in normal and AT cells exposed to high dose of low-dose-rate irradiation (2012)
  • Author(s): 24. Kawata T, Kota R, Fukada J, Shiraishi Y, Liu C, Furusawa Y, George K, Cucinotta F
  • Organizer: 58th Annual Meeting Radiation Research
  • Place of Presentation: San Juan, Puerto Rico
• [Presentation] Chromosomal aberration in normal and AT cells exposed to high dose of low-dose rate irradiation (2011)
  • Author(s): Tetsuya Kawata, et al
  • Organizer: 22nd Annual NASA Space Radiation Investigators'workshop
  • Place of Presentation: Texas USA
• [Presentation] Mechanism of potentially lethal damage repair: consideration from chromosomal aberrations. (2011)
  • Author(s): 川田 哲也他
  • Organizer: International congress of Radiation Research
  • Place of Presentation: ワルシャワ ポーランド