Regulation of reactive oxygen species induced by heavy-ion (carbon) beam; Pursuing the sophistication of heavy-ion cancer therapy

• Project/Area Number: 23591853
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: MATSUMOTO Ken-ichiro 独立行政法人放射線医学総合研究所, 重粒子医科学センター, チームリーダー (10297046)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 重粒子線がん治療 / 抗酸化剤 / レドックス制御 / 活性酸素 / フリーラジカル / 放射線 / 重粒子線 / ヒドロキシルラジカル / スーパーオキサイド / 過酸化水素 / 電子常磁性共鳴

Research Abstract

Density of radiation induced hydroxyl radical could be measured by electron paramagnetic resonance (EPR) spin trapping method. Superoxide could be measured by EPR spin probing method. Hydrogen peroxide could also be measured by EPR spin probing method. Results from those measurements gave a prediction that the very dense hydroxyl radical generation by high LET radiation can not be regulated by biologically available antioxidants concentration. Other reactive oxygen species including low density hydroxyl radical could possibly be regulated or partly regulated using antioxidants.
Inhibitory effect of TEMPOL to the radiation induced fibrosis caused by carbon-ion beam on mouse leg muscle was tested. Administration of TEMPOL dose affect to the fibrosis contrary to expectations.

Research Products

• [Journal Article] Scavenging of reactive oxygen species Induced by hyperthermia in biological fluid (2014)
  • Author(s): Ueno M, Nyui M, Nakanishi I, Anzai K, Ozawa T, Matsumoto K, Uto Y
  • Journal Title: J. Clin. Biochem. Nutr Volume: 54
  • NAID: 130004879349
  • Peer Reviewed
• [Journal Article] Scavenging of reactive oxygen species induced by hyperthermia in biological fluid (2014)
  • Author(s): 上野恵美、乳井美奈子、中西郁夫、安西和紀、小澤俊彦、松本謙一郎、宇都義浩
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 54 Issue: 2 Pages: 75-80
  • DOI: 10.3164/jcbn.13-61
  • NAID: 130004879349
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Method for Assessing X-ray-Induced Hydroxyl Radical Scavenging Activity of Biological Compounds/Materials (2013)
  • Author(s): Ueno M, Nakanishi I, Matsumoto K
  • Journal Title: J. Clin. Biochem. Nutr Volume: 52
  • NAID: 130004466712
  • Peer Reviewed
• [Journal Article] Method for assessing X-ray-induced hydroxyl radical-scavenging activity of biological compounds/materials (2013)
  • Author(s): 上野恵美
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 52 Issue: 2 Pages: 95-100
  • DOI: 10.3164/jcbn.12-75
  • NAID: 130004466712
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Temperature-Dependent Free Radical Reaction in Water (2012)
  • Author(s): Matsumoto K, Nyui M, Kamibayashi M, Ozawa T, Nakanishi I, Anzai K
  • Journal Title: J. Clin. Biochem. Nutr Volume: 50
  • NAID: 130004466665
  • Peer Reviewed
• [Presentation] Effects of TEMPOL to the Biological Results of Heavy-Ion (Carbon) (2013)
  • Author(s): Matsumoto K, Ueno M, Nakanishi I
  • Organizer: HITSRS2013
  • Place of Presentation: Chiba(ポスター)
• [Presentation] Density of hydroxyl radical generated in an aqueous solution irradiated by carbon ion beam (2012)
  • Author(s): Matsumoto K, Nakanishi I, Ueno M, Anzai K
  • Organizer: Society for Free Radical Research International 16th Biennial Meeting
  • Place of Presentation: London(ポスター)
• [Presentation] Density of hydroxyl radical generated in an aqueous solution irradiated by carbon ion beam (2012)
  • Author(s): Matsumoto K, Nakanishi I, Ueno M, Anzai K
  • Organizer: Society for Free Radical Research International 16th Biennial Meeting
  • Place of Presentation: London
• [Presentation] Distribution and Density of Hydroxyl Raidcal Generated by Heavy-Ion (Carbon) Beam Irradiation to Aqueous Sample (2011)
  • Author(s): Matsumoto K, Nakanishi I
  • Organizer: SPIN 2011 (6th International Conference on Synthesis, Properties and Implications of Nitroxides)
  • Place of Presentation: Marseille, France(ポスター)
• [Presentation] Distribution and Density of Hydroxyl Raidcal Generated by Heavy-Ion (Carbon) Beam Irradiation to Aqueous Sample (2011)
  • Author(s): Matsumoto K, Nakanishi I
  • Organizer: SPIN 2011 (6th International Conference on Synthesis, Properties and Implications of Nitroxides)
  • Place of Presentation: Marseille, France