Mechanism elucidation of proton beam specific cellular response for radiation therapy

• Project/Area Number: 23591854
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Radiation science
• Research Institution: The Wakasa Wan Energy Research Center
• Principal Investigator: HATASHITA masanori 公益財団法人若狭湾エネルギー研究センター, 研究開発部, 主任研究員 (40359237)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Hideki 福井大学, 高エネルギー医学研究センター, 准教授 (40142377)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 量子ビーム / イオンビーム / 突然変異 / 放射線感受性

Research Abstract

The differences in mutation induction and mutation spectrum between proton beams and X-rays in human cells were investigated. Cell killing effect was almost the same between proton beams and X-rays, while a difference in mutation frequency was observed and the frequency of hprt mutation of cells irradiated with proton beams was lower than that of cells irradiated with X-rays. Furthermore, the deletion spectrum of exons was different in induced mutants between proton beams and X-rays. These findings suggest that there are quantitative and qualitative differences in mutation induction between proton beams and X-rays even if the RBE values are similar.

Research Products

• [Journal Article] Sinodielide A exerts thermosensitizing effects and induces apoptosis and G2/M cell cycle arrest in DU145 human prostate cancer cells via the Ras/Raf/MAPK and PI3K/Akt signaling pathways. (2014)
  • Author(s): Masanori Hatashita, Masahiko Taniguchi, Kimiye Baba, Ken Koshiba, Takefumi Sato, Yutaka Jujo, Ryuta Suzuki, Sachiko Hayashi
  • Journal Title: International Journal of Molecular Medicine Volume: 33(2) Issue: 2 Pages: 406-414
  • DOI: 10.3892/ijmm.2013.1568
  • Peer Reviewed
• [Journal Article] 放射線応答性遺伝子発現制御システムの開発 (2013)
  • Author(s): 小川良平、森井章裕、渡部明彦、崔正国、鍵谷豪、畑下昌範、久米恭、福田茂一、長谷川崇、近藤隆
  • Journal Title: 放射線生物研究 Volume: 48(3) Pages: 267-279
  • URL: http://rbrc.kenkyuukai.jp/special/index.asp?id=11654
  • Peer Reviewed
• [Journal Article] Development of a therapeutically important radiation induced promoter. (2013)
  • Author(s): Ryohei Ogawa, Akihiro Morii, Akihiko Watanabe, Zheng-Guo Cui, Go Kagiya, Shigekazu Fukuda, Kyo Kume, Takashi Hasegawa, Masanori Hatashita, Hironori Izumi, Tetsuya Ishimoto, Loreto B. Feril. Jr.
  • Journal Title: Bioengineered. Volume: 4(1) Issue: 1 Pages: 44-49
  • DOI: 10.4161/bioe.21965
  • Peer Reviewed
• [Journal Article] Regulation of gene expression in retrovirus vectors by X-ray and proton beam radiation with artificially constructed promoters (2012)
  • Author(s): Ogawa, R., A. Morii, A. Watanabe, Z. G. Cui, G. Kagiya, S. Fukuda, K. Kume, T. Hasegawa, M. Hatashita, H. Izumi and T. Ishimoto
  • Journal Title: J Gene Med Volume: 14 Issue: 5 Pages: 316-327
  • DOI: 10.1002/jgm.2625
  • Peer Reviewed
• [Journal Article] Nitric oxide is a key molecule serving as a bridge between radiationinduced bystander and adaptive responses (2011)
  • Author(s): Matsumoto H, Tomita M, Otsuka K, Hatashita M, Hamada N
  • Journal Title: Curr.Mol.Pharmacol Volume: 4 Issue: 2 Pages: 126-134
  • DOI: 10.2174/1874467211104020126
  • Peer Reviewed
• [Presentation] Response of Human Cells to Proton Irradiation Compared to X-Ray Irradiation (2011)
  • Author(s): M. Hatashita, K. Takagi, K. Kume, G. Kagiya and H. Matsumoto
  • Organizer: The 14th International Congress of Radiation Research
  • Place of Presentation: Poland
  • Year and Date: 2011-08-30
• [Presentation] Nitric Oxide is a Key Molecule Serving as a Bridge between Radiation-Induced Bystander and Adaptive Responses. (2011)
  • Author(s): H. Matsumoto, M. Tomita, K. Otsuka, M. Maeda, N. Hamada, M. Hatashita
  • Organizer: The 14th International Congress of Radiation Research
  • Place of Presentation: Poland
  • Year and Date: 2011-08-30
• [Presentation] Response of Human Cells to Proton Irradiation Compared to X-Ray Irradiation (2011)
  • Author(s): Masanori Hatashita, Keiichi Takagi, Kyo Kume, Go Kagiya and Hideki Matsumoto
  • Organizer: The 14th International Congress of Radiation Research（ICRR2011）
  • Place of Presentation: ワルシャワ、ポーランド