Intestinal epithelial culture under an air-liquid interface

• Project/Area Number: 23591857
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Gunma University
• Principal Investigator: YOKOBORI Takehiko 群馬大学, 医学(系)研究科(研究院), 助教 (60420098)
• Co-Investigator(Kenkyū-buntansha): MIYAZAKI Tatsuya 群馬大学, 医学部附属病院, 助教 (70372349) ; TANAKA Naritake 群馬大学, 医学部附属病院, 助教 (30546726) ; KUWANO Hiroyuki 群馬大学, 大学院医学系研究科, 教授 (90186560) ; INOSE Takenori 群馬大学, 医学部, 助教 (90609968)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 食道 / 再生 / 培養 / 細胞培養 / in vitro

Research Abstract

Our study led to two new findings: (1) The intestinal epithelial culture method of Ootani et al. (Nat Med. 2009 Jun;15(6):701-706) can be applied to mouse and human esophageal cultures. (2) Esophageal organoids are rod-like luminal structures lined with fibroblasts, and regeneration of the esophageal mucosal surface can be almost completely achieved in vitro.
The method described in the generation of long-term cultures of human esophageal cells without the need for gene transfer for immortalization. Due to autologous myofibroblasts from a collected specimen are used as a niche, feeder cells are not required for plating. In addition, the matrix gel, in which cells are embedded, and the culture medium are commercially available. These merits are important for clinical application of our method.Therefore, we believe that this new method has the potential to be an important tool to advance research in intestinal biology, carcinogenesis, and regenerative medicine in both mice and humans.

Research Products

• [Journal Article] FBXW7 mediates chemotherapeutic sensitivity and prognosis in NSCLCs (2014)
  • Author(s): Yokobori T, Yokoyama Y, Mogi A, Endoh H, Altan B, Kosaka T, Yamaki E, Yajima T, Tomizawa K, Azuma Y, Onozato R, Miyazaki T, Tanaka S, Kuwano H
  • Journal Title: Mol Cancer Res Volume: 12(1) Pages: 32-7
  • Peer Reviewed
• [Journal Article] FBXW7 mediates chemotherapeutic sensitivity and prognosis in NSCLCs. (2014)
  • Author(s): Yokobori T, Yokoyama Y, Mogi A, Endoh H, Altan B, Kosaka T, Yamaki E, Yajima T, Tomizawa K, Azuma Y, Onozato R, Miyazaki T, Tanaka S, Kuwano H.
  • Journal Title: Mol Cancer Res. Volume: 12(1) Issue: 1 Pages: 32-7
  • DOI: 10.1158/1541-7786.mcr-13-0341
  • Peer Reviewed
• [Journal Article] Nuclear karyopherin-α2 expression in primary lesions and metastatic lymph nodes was associated with poor prognosis and progression in gastric cancer (2013)
  • Author(s): Altan B, Yokobori T, Mochiki E, Ohno T, Ogata K, Ogawa A, Yanai M, Kobayashi T, Luvsandagva B, Asao T, Kuwano H
  • Journal Title: Carcinogenesis Volume: 34(10) Pages: 2314-21
  • Peer Reviewed
• [Journal Article] MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB1 (2013)
  • Author(s): Yokobori T, Suzuki S, Tanaka N, Inose T, Sohda M, Sano A, Sakai M, Nakajima M, Miyazaki T, Kato H, Kuwano H
  • Journal Title: Cancer Sci Volume: 104(1) Pages: 48-54
  • Peer Reviewed
• [Journal Article] Nuclear karyopherin-α2 expression in primary lesions and metastatic lymph nodes was associated with poor prognosis and progression in gastric cancer. (2013)
  • Author(s): Altan B, Yokobori T, Mochiki E, Ohno T, Ogata K, Ogawa A, Yanai M, Kobayashi T, Luvsandagva B, Asao T, Kuwano H.
  • Journal Title: Carcinogenesis. Volume: 34 Issue: 10 Pages: 2314-2321
  • DOI: 10.1093/carcin/bgt214
  • Peer Reviewed
• [Journal Article] MiR-150 is associated with poor prognosis in esophageal squamous cell carcinoma via targeting the EMT inducer ZEB1. (2013)
  • Author(s): Yokobori T
  • Journal Title: Cancer Sci. Volume: 104 Issue: 1 Pages: 48-54
  • DOI: 10.1111/cas.12030
  • Peer Reviewed
• [Journal Article] Copy number loss of FBXW7 is related to gene expression and poor prognosis in esophageal squamous cell carcinoma (2012)
  • Author(s): Yokobori T, Mimori K, Iwatsuki M, Ishii H, Tanaka F, Sato T, Toh H, Sudo T, Iwaya T, Tanaka Y, Onoyama I, Kuwano H, Nakayama KI, Mori M
  • Journal Title: Int J Oncol Volume: 41(1) Pages: 253-9
  • Peer Reviewed
• [Journal Article] TNFAIP8 overexpression : clinical relevance to esophageal squamous cell carcinoma (2012)
  • Author(s): Hadisaputri YE, Miyazaki T, Suzuki S, Yokobori T, Kobayashi T, Tanaka N, Inose T, Sohda M, Kuwano H
  • Journal Title: Ann Surg Oncol Volume: 3(Suppl)
  • Peer Reviewed
• [Presentation] 食道のin vitro培養法・マウスへの移植手技確立にむけて (2014)
  • Author(s): 横堀武彦
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
• [Presentation] マウス肺組織のin vitro培養法、移植法の確立にむけて (2013)
  • Author(s): 横堀武彦
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] マウス、ヒト食道組織のin vitro培養法の確立-先進的再生医療にむけて- (2012)
  • Author(s): 横堀武彦
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 幕張シンポジウム
• [Presentation] 食道組織のin vitro培養 (2012)
  • Author(s): 横堀武彦
  • Organizer: 第8回日本消化管学会
  • Place of Presentation: 仙台シンポジウム
• [Presentation] 食道組織のin vitro培養 (2012)
  • Author(s): 横堀武彦
  • Organizer: 第8回日本消化管学会 ワークショップ2「消化管stem cellリサーチの進歩」
  • Place of Presentation: 仙台
• [Presentation] マウス、ヒト食道組織のin vitro培養法の確立 －先進的再生医療にむけて－
  • Author(s): 横堀武彦
  • Organizer: 第112回日本外科学会定期学術集会 シンポジウム11. 外科領域における先端技術・治療の開発
  • Place of Presentation: 千葉（幕張メッセ）