Reconstruction of human liver sheet for preclinical study of liver regenerative medicine
| Project/Area Number |
23591868
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
EGUCHI Susumu 長崎大学, 医歯薬学総合研究科(医学系), 教授 (80404218)
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| Co-Investigator(Kenkyū-buntansha) |
KANEMATSU Takashi 長崎大学, 医歯薬学総合研究科(医学系), 名誉教授 (40128004)
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| Research Collaborator |
SAKAI Yusuke 長崎大学, 医歯薬学総合研究科(医学系), 助教 (10608904)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 再生医療 / 肝臓 / 細胞シート / ヒト / 移植 / 肝細胞 |
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| Outline of Final Research Achievements |
The liver regenerative medicine is extremely-difficult to establish, and the further research is looked forward to. In this study, we describe a novel liver regenerative medicine technology using reconstructed human liver sheet.
Human primary hepatocytes with more than 80 % of viability were isolated from surgical liver segment. Hepatocyte-only sheets were constructed by culturing it in suitable cell density. The engineered hepatocytes/fibroblast sheets (EHFSs) were constructed by culturing hepatocytes or HepaRG cells (well-differentiated hepatic cells derived from a human liver tumor) on a fibroblast. The EHFSs showed superior expression levels of vascularization-associated growth factors (VEGF, TGFb1, and HGF) in vitro. In addition, the EHFSs formed vascularized subcutaneous human liver tissues in mice. This technology could be expected as novel liver regenerative medicine technology.
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Report
(5 results)
Research Products
(42 results)
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[Presentation] Rapid fabrication of engineered liver tissue using novel fibroblast system2014
Author(s)
Yusuke Sakai, Makiko Koike, Hideko Hasegawa, Kosho Yamanouchi, Akihiko Soyama, Masaaki Hidaka, Mitsuhisa Takatsuki, Tamotsu Kuroki, Teruo Okano, and Susumu Eguchi
Organizer
9th World Congress on Alternatives and Animal Use in the Life Sciences (WC9)
Place of Presentation
Prague, Czech Republic
Year and Date
2014-08-24 – 2014-08-28
Related Report
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[Presentation] Induction of hepatic progenitor cells by transplanted rat dermal fibroblast allograft sheet on an incised rat liver.2011
Author(s)
Muraoka I, Soyama A, Takatsuki M, Tomonaga T, Hidaka M, Kanematsu T,Hishikawa Y, Koji T, Utoh R, Ohashi K, Eguchi S.
Organizer
62nd AASLD
Place of Presentation
SF, USA
Related Report
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