| Project/Area Number |
23591873
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
YOSHIMURA NORIO 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00191643)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUYAMA Masahide 京都府立医科大学, 医学研究科, 講師 (80549767)
鈴木 智之 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80705450)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | マイクロキメリズム / IL-10 / マウス異所性心移植モデル / 免疫寛容 |
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| Research Abstract |
(AIMS)We investigated that transduction of the human Interleukin-10 (IL-10) gene in mouse foetal liver stem cells (hIL-10-TFLs) protects them from rejection in an allogeneic host and the effects of these cells in organ transplantation.(METHODS)Balb/c mice were sublethally irradiated before receiving vascularized heterotopic heart grafts from C57BL/6 mice. TFLs from C57BL/6 mice transduced with hIL-10, were injected on the day of transplantation, every 20 days.(RESULTS)Heart transplants survived 86.25 versus 46.3 or 11.5 days, in mice which received repeated injections, a single injection, or in control mice. Expressions of H-2b and hIL-10 were found in thymus, liver. Foxp3+ cells present inside the transplanted heart.(CONCLUSIONS)We have shown that repeated injections of hIL-10-TFLs are efficient in impairing transplant rejection. This"prope tolerance"was associated with survival of donor haematopoietic cells in the host.
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