| Project/Area Number |
23591904
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
YOKOYAMA Shozo 和歌山県立医科大学, 医学部, 講師 (90398462)
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| Co-Investigator(Kenkyū-buntansha) |
HOTTA Tsukasa 和歌山県立医科大学, 医学部, 講師 (50244744)
TAKIFUJI Katsunari 和歌山県立医科大学, 医学部, 講師 (00254540)
MATSUDA Kenji 和歌山県立医科大学, 医学部, 助教 (30398458)
YAMAUE Hiroki 和歌山県立医科大学, 医学部, 教授 (20191190)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | CEACAM1 / 大腸癌 / 癌幹細胞様特性 / 抗癌剤感受性 / 腺管形成 / 癌幹細胞 |
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| Research Abstract |
Enhanced CEACAM-4S (short cytoplasmic isoform) induced CD44 expression, incresed ALDH1 activity, and anchorage indeoendent growth of colorectal cancer cells compared with vector control, suggesting that a possibility that CEACAM1-4S induce CSC property. In clinical setting, Overexpression of CEACAM1-S (short cytoplasmic isoform) at the invasion fronat of colorectal cancer tissue indicated poor survival of patients. By addition of 5FU to colorectal cancer cells on plastic, CEACAM1-4L transfected HT29 cells significantly survived in comparison with CEACAM1-4S or vector control transfected cells. In 3D culture, addition of 5FU induced apoptotic burst of colorectal cancer sphere formed by CEACAM1-4S. A multivariate analysis for recurrence after curative surgery and adjuvant chemotherapy for Stage III colorectal cancer patients showed that the hollow spheroid formation beyond the invasive front is an independent risk factor.
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