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Basic and clinical research of circulating DNA fragments as a biomarker in gastroenterological cancer.

Research Project

Project/Area Number 23591907
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionTokai University

Principal Investigator

OZAWA Soji  東海大学, 医学部, 教授 (10169287)

Co-Investigator(Renkei-kenkyūsha) MIYACHI Hayato  東海大学, 医学部, 教授 (20174196)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords癌 / 核酸 / 外科 / 遺伝子 / 食道癌 / 手術 / 化学療法 / バイオマーカー
Research Abstract

Human squamous cancer cells (TE-4) were inoculated subcutaneously in the bilateral back of BALB/c nude mice. Unilateral tumors or bilateral tumors were resected 3 weeks after inoculation as surgical models. And 5-FU (25mg/kg or 50mg/kg) was injected intraperitoneously 4 times 3 weeks after inoculation as chemotherapy models. Blood was collected and animals were sacrificed 5 weeks after inoculation. Serum was processed and used for DNA measurement using a real time PCR method. DNA concentrations (Alu 247 and Alu115) correlated with tumor volumes. Serum DNA seems to be a good biomarker for cancer staging and evaluating chemotherapeutic effects.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (1 results)

All 2011

All Presentation (1 results)

  • [Presentation] Highly sensitive diagnostic method for colorectal cancer using the ratio of free DNA fragments in the serum2011

    • Author(s)
      ArakawaS, Ozawa S, Ando T, Takeuchi H, Kitagawa Y, Umemoto S, Morise Z
    • Organizer
      International Surgical Week 2011
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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