Basic and clinical research of circulating DNA fragments as a biomarker in gastroenterological cancer.

• Project/Area Number: 23591907
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Tokai University
• Principal Investigator: OZAWA Soji 東海大学, 医学部, 教授 (10169287)
• Co-Investigator(Renkei-kenkyūsha): MIYACHI Hayato 東海大学, 医学部, 教授 (20174196)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 癌 / 核酸 / 外科 / 遺伝子 / 食道癌 / 手術 / 化学療法 / バイオマーカー

Research Abstract

Human squamous cancer cells (TE-4) were inoculated subcutaneously in the bilateral back of BALB/c nude mice. Unilateral tumors or bilateral tumors were resected 3 weeks after inoculation as surgical models. And 5-FU (25mg/kg or 50mg/kg) was injected intraperitoneously 4 times 3 weeks after inoculation as chemotherapy models. Blood was collected and animals were sacrificed 5 weeks after inoculation. Serum was processed and used for DNA measurement using a real time PCR method. DNA concentrations (Alu 247 and Alu115) correlated with tumor volumes. Serum DNA seems to be a good biomarker for cancer staging and evaluating chemotherapeutic effects.

Research Products

• [Presentation] Highly sensitive diagnostic method for colorectal cancer using the ratio of free DNA fragments in the serum (2011)
  • Author(s): ArakawaS, Ozawa S, Ando T, Takeuchi H, Kitagawa Y, Umemoto S, Morise Z
  • Organizer: International Surgical Week 2011
  • Place of Presentation: パシフィコ横浜