| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Research Abstract |
We established several pancreatic cancer models using hamster pancreatic cancer cell line, PGHAM-1. PGHAM-1 cells with luciferase activity transduced by viral vectors (PGHAM-1-Luc) ware used for in vivo imaging of cancer proliferation and metastasis using IVIS system by measuring the bioluminescent signals. Using this system, the effect of gemcitabine on pancreatic cancer model was investigated in vitro and in vivo. Anti-tumor effect of gemcitabine was found in vitro but not in vivo by using IVIS system quantitative analysis. This cancer model is suitable for detecting the new therapeutic agents, although improved in vivo imaging system and its application are needed in the near future.
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