Novel treatment for triple negative breast cancer using Poloxamer

• Project/Area Number: 23591912
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General surgery
• Research Institution: Kurume University
• Principal Investigator: IWAKUMA Nobutaka 久留米大学, 医学部, 助教 (30309801)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 癌ナノテクノロジー / トリプルネガティブ乳癌 / ミセルナノ粒子 / 乳癌

Outline of Final Research Achievements

Poloxamer was toxic to triple negative human breast cancer cells MDA-MB-231 in culture as evidenced by propidium iodide assays compared with human mammary epithelial (non-cancer) cells MCF12A. Poloxamer had significant cytotoxicity against MDA-MB-231 than MCF12A by the LDH assay. Poloxamer was not associated with cell proliferation in both MDA-MB-231 and MCF12A.
oPloxamer inhibited the growth of breast cancer tumors in intravenous administration. However, Ploxamer did not inhibit the growth of breast cancer tumors in the oral administration.

Research Products

• [Journal Article] HER2-targeted Therapeutic Strategies for HER2-positive Metastatic Breast Cancers (2014)
  • Author(s): Teruhiko Fujii, Keisuke Miwa, Tomoyuki Ushijima, Mototsugu Matsunaga, Masaru Fukahori, Kotaro Yuge, Uhi Toh, Nobutaka Iwakuma, Ryuji Takahashi, Hiroki Takahashi, Miki Takenaka, Mai Mishima, Yoshito Akagi, Masayoshi Kage , Shino Nakagawa
  • Journal Title: Current Cancer Therapy Reviews Volume: 10 Issue: 3 Pages: 225-233
  • DOI: 10.2174/157339471003150212111019
  • Peer Reviewed / Open Access
• [Journal Article] 乳がん診療へのがんナノテクノロジー研究の応用 (2013)
  • Author(s): 岩熊伸高、唐 宇飛、高良慶子、三島麻衣、竹中美貴、高橋龍司、白水和雄、S.R.Grobmyer
  • Journal Title: 臨牀と研究 Volume: 90巻81 Pages: 81-85
  • Peer Reviewed
• [Journal Article] FOXP3 expression in tumor cells and tumor-infiltrating lymphocytes is associated with breast cancer prognosis (2013)
  • Author(s): Miki Takenaka, Naoko Seki, Uhi Toh, Satoshi Hattori, Akihiko Kawahara, Tomohiko Yamaguchi, Keiko Koura, Ryuji Takahashi, Hiroko Otsuka, Hiroki Takahashi, Nobutaka Iwakuma, Shino Nakagawa, Teruhiko Fujii, Tetsuro Sasada, Rin Yamaguchi, Hirohisa Yano, Kazuo Shirouzu, Masayoshi Kage
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Issue: 4 Pages: 625-632
  • DOI: 10.3892/mco.2013.107
  • Peer Reviewed
• [Journal Article] 乳がん診療へのがんナノテクノロジー研究の応用 (2013)
  • Author(s): 岩熊 伸高、唐 宇飛、高良 慶子、三島 麻衣、竹中 美貴、高橋 龍司、白水 和雄、Stephen R Grobmyer
  • Journal Title: 臨牀と研究 Volume: 90 Pages: 81-85
• [Journal Article] 機能的イメージングを応用した治療の個別化－ナノテクノロジーによる乳がんの診断と治療 (2012)
  • Author(s): 岩熊伸高、Stephen R Grobmyer、唐 宇飛、高良慶子、白水和雄
  • Journal Title: 腫瘍内科 Volume: 10 Pages: 131-135
• [Presentation] ナノ粒子を用いた乳がん腫瘍組織へのドラッグデリバリー量的解析 (2014)
  • Author(s): 岩熊伸高、唐 宇飛、Grobmyer Stephen、三島麻衣、竹中美貴、古川実奈、岩本周子、藤井輝彦、赤木由人、白水和雄、淡河恵津世
  • Organizer: 第22回日本乳癌学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-07-10 – 2014-07-12
• [Presentation] ポリヒドロキシフラーレンを用いた非侵襲的イメージングと新しい乳癌局所治療に関する研究 (2014)
  • Author(s): 岩熊伸高、唐 宇飛、Grobmyer Stephen、Krishna Vijay、三島麻衣、竹中美貴、高橋龍司、高良慶子、藤井輝彦、白水和雄
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-03 – 2014-04-05
• [Presentation] STUDY OF PATIENTS WITH STAGE III BREAST CANCER WHO RECEIVED NEOADJUVANT CHEMOTHERAPY (2013)
  • Author(s): N.Iwakuma,U.Toh,R.Takahashi.M.Mishima,M.Takenaka,K.Koura,T.Fujii,K.Shirouzu
  • Organizer: PRIMARY THERAPY OF EARLY BREAST CANCER 13TH INTERNATIONAL CONFERENCE
  • Place of Presentation: ザンクトガレン、スイス
• [Presentation] 転移性乳癌に対するナノ粒子デリバリー (2013)
  • Author(s): 岩熊伸高、唐 宇飛、三島麻衣、竹中美貴、高橋龍司、高良慶子、Grobmyer Stephan、藤井輝彦、白水和雄
  • Organizer: 第21回日本乳癌学会学術集会
  • Place of Presentation: 浜松
• [Presentation] Targeting nanoparticle: A new material in breast oncology (2012)
  • Author(s): N. Iwakuma, S. R. Grobmyer, U. Toh, K. Koura, H. Otsuka, R. Takahashi, M. Takenaka, K. Shirouzu
  • Organizer: 第20回日本乳癌学会学術総会
  • Place of Presentation: 熊本
• [Presentation] Active targeting nanoparticle for breast cancer diagnosis and treatment (2012)
  • Author(s): N. Iwakuma, S. R. Grobmyer, U. Toh, K. Koura, M. Mishima, M. Takenaka, H. Otsuka, R. Takahashi, K. Shirouzu
  • Organizer: 2012 Breast Cancer Symposium
  • Place of Presentation: San Francisco, USA
• [Presentation] 乳癌克服のための癌ナノテクノロジー研究の役割と今後の展望 (2011)
  • Author(s): 岩熊伸高
  • Organizer: 第19回日本乳癌学会学術総会
  • Place of Presentation: 仙台国際センター（宮城）