| Project/Area Number |
23591926
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Mie University |
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Principal Investigator |
YASUDA Hiromi 三重大学, 医学部附属病院, 助教 (60586767)
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| Co-Investigator(Kenkyū-buntansha) |
TOIYAMA Yuji 三重大学, 医学部附属病院, 助教 (00422824)
INOUE Yasuhiro 三重大学, 医学部附属病院, 講師 (20324535)
KUSUNOKI Masato 三重大学, 医学部附属病院, 教授 (50192026)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | microRNA / エピジェネティック / 消化器癌 / 胃癌 / 腹膜播種 / マイクロRNA / メチル化解析 / cDNA解析 |
|---|
| Research Abstract |
Distant metastasis tissues showed higher expression of miR-200c and miR-141 than did primary GICs, which was significantly associated with hypomethylation of the promoter region of these miRNAs. Transfection of miR-200c precursors resulted in enhanced cell proliferation but reduced invasion and migration behaviours in cancer cell lines, and caused reduced expression of putative gene targets, and resulted in epithelial-mesenchymal transition. In addition, high serum miR-200c demonstrated a significant positive correlation with lymph node metastasis, distant metastasis, and prognosis. More importantly, serum miR-200c was an independent predictor for lymph node metastasis and tumor recurrence and emerged as an independent prognostic marker.
In conclusion, miR-200c, epigenetically regulated, plays an important role in mediating EMT and metastatic behaviour in GIC. Serum miR-200c has strong potential to serve as a noninvasive biomarker for GIC prognosis and predicting metastasis.
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