Quantitative detection of circulating tumor DNA in the plasma of gastric cancer patients

• Project/Area Number: 23591928
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Osaka University
• Principal Investigator: KUROKAWA Yukinori 大阪大学, 医学(系)研究科(研究院), 助教 (10470197)
• Co-Investigator(Kenkyū-buntansha): TAKIGUCHI Shuji 大阪大学, 医学系研究科, 講師 (00301268) ; KATO Kikuya 大阪府立成人病センター研究所, 所長 (60194809) ; 谷口 一也 地方独立行政法人大阪府立病院機構大阪府立成人病センター(研究所), その他部局等, その他 (70463289)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 胃癌 / ctDNA / BEAMing / 次世代シーケンサー

Research Abstract

We performed quantitative detection of circulating tumor DNA (ctDNA) that harbors gene mutations identical to the primary tumor, by analyzing cell-free DNA in the plasma of gastric cancer patients using BEAMing or next generation sequencer. 3 out of 6 advanced gastric cancer patients showed positive ctDNA for TP53 before surgery. ctDNA level corresponded to surgical resection, tumor recurrence, and response to chemotherapy. We demonstrated that ctDNA might be useful biomarker for monitoring tumor progression in gastric cancer patients.

Research Products

• [Presentation] Circulating tumor DNA can be useful biomarker for gastric cancer by full utilization of next generation sequencer (2013)
  • Author(s): Takuya Hamakawa, Yoji Kukita, Yukinori Kurokawa, et al.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2013-10-03
• [Presentation] 次世代シーケンサーを用いた胃癌患者血漿中の癌細胞由来遊離DNAの検出 (2013)
  • Author(s): 浜川卓也、久木田洋児、黒川幸典ほか
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2013-04-11
• [Presentation] 次世代シーケンサーを用いた胃癌患者血漿中の癌細胞由来遊離DNAの検出 (2013)
  • Author(s): 浜川卓也、黒川幸典ほか
  • Organizer: 第113回 日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] 次世代シーケンサーを用いた胃癌患者血漿中の癌細胞由来遊離DNAの検出 (2012)
  • Author(s): 浜川卓也、久木田洋児、黒川幸典ほか
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-20
• [Presentation] 次世代シーケンサーを用いた胃癌患者血漿中の癌細胞由来遊離DNAの検出
  • Author(s): 浜川卓也、黒川幸典、ほか
  • Organizer: 第71回 日本癌学会学術総会
  • Place of Presentation: 札幌