| Project/Area Number |
23591939
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | 独立行政法人国立国際医療研究センター (2012-2013)
Kyushu University (2011) |
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Principal Investigator |
TSUJITANI Shunichi 独立行政法人国立国際医療研究センター, その他部局等, その他 (30188544)
|
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| Co-Investigator(Kenkyū-buntansha) |
KEKEJI Yoshihiro 神戸大学, 大学院食道胃腸外科, 教授 (80284488)
MORIT Masaru 国立病院機構九州がんセンター, 消化器外科, 部長 (30294937)
KOHNOE Shunji 田川市立病院, 院長 (30215199)
EGASHIR Akinori 国立病院機構九州がんセンター, 消化器外科 (00419524)
SONODA Hideto 伊万里有田共立病院, 外科, 医長 (00465725)
OHGA Takefumi 国立病院機構福岡東医療センター, 外科, 部長 (60335958)
|
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | がん幹細胞 / 免疫回避機構 / 樹状細胞 / 免疫抑制 / 胃癌 / 腹膜播種 |
|---|
| Research Abstract |
Peritoneal dissemination of gastric cancer stem cells and immunotherapy overcoming immune escape were investigated. Cultured gastric cancer cell lines MKN45 and MKN74 were sorted by FACS int0 CD44+ and CD44- cells. CD44+ gastric cancer cells expressed TGF-b , IL-10 and VEGF. Therefore, gastric cancer stem cells may have high potential to escape from immune surveillance. However, CTL from healthy donor co-cultured with CD44+ gastric cancer cells could kill CD44+ gastric cancer cells as well as that CTL co-cultured with CD44- gastric cancer cells could kill CD44- gastric cancer cells. These results indicate that cancer stem cells may not be resistant to immunotherapy but the cancer stem cells may be protected by strong escape mechanisms provided by host cells.
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