| Project/Area Number |
23591958
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Mie University |
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Principal Investigator |
UCHIDA Keiichi 三重大学, 医学部附属病院, 准教授 (30293781)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Mikihiro 三重大学, 医学部附属病院, 助教 (30422835)
MIZOGUCHI Akira 三重大学, 大学院医学系研究科, 教授 (90181916)
KUSUNOKI Masato 三重大学, 大学院医学系研究科, 教授 (50192026)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 潰瘍性大腸炎 / 二光子レーザー顕微鏡 / DSS腸炎マウス / 炎症性腸疾患 |
|---|
| Research Abstract |
In this study, we attempted to visualize in vivo therapeutic response of infliximab (IFX) in the murine UC model using two photon laser scanning microscopy (TPLSM) and to analyze their histopathological response morphologically. Both extensive inflammatory cell infiltration and the damage or loss of epithelial cells in the crypt was observed in vivo real-time in the dextran sodium sulfate (DSS) colitis model of green fluorescent protein (GFP) expressing mice using TPLSM. The number of leukocyte infiltrates within the crypt (2.4 versus 6 per field of view: FOV) and the number of crypt loss (1.0 versus 4.5 per FOV) were reduced in IFX-treated mice, compared with non-IFX, control mice. The length of colonic crypt (99.8 versus 80.0 micrometer) was increased in IFX-treated mice, compared with non-IFX, control mice. Serial change in regenerative epithelial cells was not observed, which regenerated from the bottom of the crypt toward the top of the crypt (the luminal side).
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