New method for ulcerative colitis associated cancer

• Project/Area Number: 23591960
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: ARAKI Toshimitsu 三重大学, 医学部附属病院, 講師 (70343217)
• Co-Investigator(Kenkyū-buntansha): KUSUNOKI Masato 三重大学, 大学院医学系研究科, 教授 (50192026) ; YOSHIYAMA Shigeyuki 三重大学, 医学部附属病院, 診療従事者 (60444436) ; OKITA Yoshiki 三重大学, 医学部, 助教 (20378342) ; MIKI Chikao 三重大学, 大学院医学系研究科, 客員教授 (50242962)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 小腸大腸肛門外科学 / 潰瘍性大腸炎 / 大腸癌 / 異形成 / 一酸化窒素 / 酸化 / 炎症性発癌

Research Abstract

To clarify whether the expression of nitrative and oxidative DNA damage markers in the rectal mucosa of patients with ulcerative colitis (UC) could be used to predict UC-associated neoplasia.
A total of 141 patients with UC who underwent a proctocolectomy were enrolled in this study. The expression of 8-nitroguanine (8-NG), 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG), and inducible nitric oxide synthase (iNOS) in the rectal mucosa were evaluated using immunohistochemistry (IHC) and assessed relative to the pathogenesis of UC-associated neoplasia.
The results suggest that evaluating the expression levels of 8-NG in the rectal mucosa may be a useful biomarker for detecting patients with UC-associated neoplasia.

Research Products

• [Journal Article] Identification of patients with developing ulcerative colitis-associated neoplasia by nitrative DNA damage marker 8-nitroguanin expression in rectal mucosa (2013)
  • Author(s): Saigusa S, Araki T, Tanaka K, Hashimoto K, Okita Y, Fujikawa H, Okugawa Y, Toiyama Y, Inoue Y, Uchida K, Mohri Y, Kusunoki M.
  • Journal Title: J Clin Gastroenterol Volume: 47
  • Peer Reviewed
• [Journal Article] Identification of patients with developing ulcerative colitis-associated neoplasia by nitrative DNA damage marker 8-nitroguanin expression in rectal mucosa. (2013)
  • Author(s): Saigusa S, Araki T, Tanaka K, Hashimoto K, Okita Y, Fujikawa H, Okugawa Y, Toiyama Y, Inoue Y, Uchida K, Mohri Y, Kusunoki M.
  • Journal Title: J Clin Gastroenterol Volume: 47 Issue: 9 Pages: e80-e86
  • DOI: 10.1097/mcg.0b013e31828f51e1
  • Peer Reviewed
• [Presentation] 一酸化窒素の発癌機構に着目したcolitic cancer早期診断アプローチ (2012)
  • Author(s): 荒木俊光, 三枝晋, 橋本清, 大北喜基, 藤川裕之, 奥川喜永, 川村幹雄, 廣純一郎, 田中光司, 井上靖浩, 内田恵一, 毛利靖彦, 楠正人
  • Organizer: 第67回日本大腸肛門病学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2012-11-17