Analysis of amino acid-signal transduction in the intestinal system, applying a new nipple-bottle feeding system and artificial milk formula for newborn mice.

• Project/Area Number: 23591976
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Kawasaki Medical School
• Principal Investigator: YOSHIDA KAZUHIRO 川崎医科大学, 医学部, 講師 (10220633)
• Co-Investigator(Kenkyū-buntansha): YAMATSUJI Tomoki 川崎医科大学, 医学部, 准教授 (40379730)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アミノ酸 / 腸管細胞増殖因子 / 小腸大腸肛門外科学

Research Abstract

We have presented that feeding neonatal mice with glutamine-deleted artificial milk formula induced high incidence of colonic hemorrhage due to the epithelial cell death, applying a new nipple-bottle feeding system for newborn mice. IEC6 rat intestinal epithelial cells cultured with low concentration of glutamine underwent apoptosis.
Glutamine deprivation might cause instability of the intestinal epithelial alignment by apoptosis.

Research Products

• [Journal Article] The role s of glutamine, leucine and arginine (2014)
  • Author(s): Kazuhiro YOSHIDA, Yoshio NAOMOTO, Tomoki YAMATSUJI, Kaori SHIGEMITSU, Munen ori TAKAOKA, Takuya FUKAZAWA, Naomasa ISHIDA, Jiro HAYASHI, Yohko HIRABAYASHI, Astushi URAKAMI, Kazutaka NAKASHIMA, Ichiro MORITA and Minoru HAISA, Amino ac id signaling in the intestine
  • Journal Title: Kawasaki Medical Journal Volume: (掲載予定)