Development of artificial liver support system combining with liver cells and Kuppfer cells.
| Project/Area Number |
23591984
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | University of Yamanashi |
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Principal Investigator |
FUJII Hideki 山梨大学, 医学工学総合研究部, 教授 (30181316)
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| Co-Investigator(Kenkyū-buntansha) |
KONO Hiroshi 山梨大学, 医学工学総合研究部, 助教 (40322127)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 肝不全 / 人工肝補助装置 / 肝Kupffer細胞 / GCSF / M-CSF / 新生血管 / IL-17 / 脾臓摘出 / Kupffer細胞 / ノックアウトマウス / 肝再生 / TNF-α / CD4陽性リンパ球 / CLP model / IL-17A / HMGB1 / 肝温虚血再還流モデル / glycyrrhizin |
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| Research Abstract |
IL-17A regulates neutrophil-dependent organ injury. The purpose of this study was to determine the role of IL-17A in neutrophil recruitment after ischemia-reperfusion (I/R). Wild-type (WT) and IL-17A knockout (KO) mice were evaluated. In another experiments, recombinant (rm) IL-17A or rmIL-17A/F were administered before I/R. Isolated Kupffer cells were incubated with rmIL-17A and production of TNF-alpha was measured. The extent of liver injury demonstrated similar levels in the acute phase (6 h) in these two models. In contrast, in the subacute phase (20 h), liver injury were significantly reduced in the KO compared with the WT. This reduction in liver injury was associated with suppression of chemokine, adhesion molecule expression and reduction in infiltration of neutrophils into the liver. TNF-alpha production by isolated Kupffer cells increased significantly with rmIL-17A. IL-17A is a key regulator in initiating neutrophil-induced inflammatory responses and hepatic injury.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Early hepatocellular carci nomas showing isointensity or hyper intensity in gadoxetic Acid-enhance d, hepatocyte-phase magnetic resona nce images2013
Author(s)
Chen N, Motosugi U, Sano K, Ichikaw a T, Nakano M, Morisaka H, Ichikawa S, Matsuda M, Fujii H, Enomoto N, Araki T.
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Journal Title
J Comput Assist Tomogr
Volume: 37
Issue: 3
Pages: 466-469
DOI
Related Report
Peer Reviewed
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[Journal Article] Y.Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells.2012
Author(s)
Osada, M., Inoue, O., Ding, G., Shirai, T., Ichise, H., Hirayama, K., Takano, K., Yatomi, Y., Hirashima, M., Fujii, H., Suzuki-Inoue, K., Ozaki
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Journal Title
The Journal of biological chemistry.
Volume: 287
Issue: 26
Pages: 22241-22252
DOI
Related Report
Peer Reviewed
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