• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Tumor suppressor FHIT regulates tumor invasion of pancreatic cancer cells via inhibiting association of Ezrin and Actin cytoskeleton

Research Project

Project/Area Number 23592009
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Digestive surgery
Research InstitutionOkayama University

Principal Investigator

NISHIZAKI Masahiko  岡山大学, 大学病院, 助教 (30379789)

Co-Investigator(Kenkyū-buntansha) FUJIWARA Toshiyoshi  岡山大学, 大学院医歯薬学総合研究科, 教授 (00304303)
Co-Investigator(Renkei-kenkyūsha) KAGAWA Shunsuke  岡山大学, 病院, 講師 (00362971)
NAGASAKA Takeshi  岡山大学, 病院, 助教 (30452569)
TAZAWA Hiroshi  岡山大学, 助教 (90415513)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords遺伝子治療 / 癌 / 転移・浸潤 / FHIT / PKC / 膵癌
Research Abstract

Ezrin is recognized as a key component in tumor metastasis and regulated by PKC. Abnormalities of tumor suppressor FHIT, whitch shares homology with PKCI (PKC-interacting protein), have been found in pancreatic cancers. In this study, we showed that FHIT overexpression in S2-VP10 pancreatic adenocarcinoma cells by transfection of Ad- FHIT suppressed migratory/invasive capacities of tumor cells. We observed that overexpression of FHIT largely reduced threonine-phosphorylated Ezrin. Furthermore, FHIT overexpression interrupted not only Ezrin and Actin cytoskeleton interaction but also Ezrin and PKC alpha interaction. We also found Fhit and PKC alpha binding in the immunoprecipitation analysis. Ectopic activation of FHIT significantly reduced the invasive potential in S2-VP10 cells. These findings suggested that overexpression of FHIT protein might regulate tumor invasion via inhibiting Ezrin and Actin cytoskeleton interaction.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (2 results)

All 2013

All Presentation (2 results)

  • [Presentation] Tumor suppressor FHIT regulates tumor invasion of pancreatic cancer cells via inhibiting association of Ezrin and Actin cytoskeleton2013

    • Author(s)
      西崎正彦, 香川俊輔, 田澤大, 藤原俊義, 黒田新士, 岸本浩行
    • Organizer
      第19回日本遺伝子治療学会
    • Place of Presentation
      ホテルグランヴィア岡山
    • Year and Date
      2013-07-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Tumor suppressor FHIT regulates tumor invasion of pancreatic cancer cells via inhibiting association of Ezrin and Actin cytoskeleton2013

    • Author(s)
      西崎正彦 田澤大 黒田新士 岸本浩行 香川俊輔 藤原俊義
    • Organizer
      第19回日本遺伝子治療学会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi