Identification of pancreatic stellate cell associated-microRNA that suppress tumor-stromal interaction and clarification of its mechanism.
| Project/Area Number |
23592012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
TOMINAGA Yohei 九州大学, 医学(系)研究科(研究院), 共同研究員 (90304823)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Norihiro 産業医科大学, 医学(系)研究科(研究院), 助教 (20423527)
UEDA Jynji 九州大学, 大学病院, 助教 (90529801)
NABAE Toshinaga 九州大学, 医学研究院, 共同研究員 (10467889)
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| Co-Investigator(Renkei-kenkyūsha) |
MIZUMOTO Kazuhiro 九州大学, 大学病院, 准教授 (90253418)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 膵癌 / microRNA / miRNA / IPMN |
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| Research Abstract |
We analyzed microRNAs of pancreatic stellate cells(PSCs) derived from pancreatic cancer and normal pancreas. Microarray showed miR-31 and miR-34a increased in cancer associated PSCs and miR-21 decreased compared with normal PSCs. Next, we established highly metastatic pancreatic cancer cells to identify the miRs that associate with metastasis. In highly metastatic SUIT-2 cells(MSCs), microarray showed that miR-125b-5p, miR-4324 and miR-4706 increased and miR-16-5p, miR-21-5p, miR-192, miR-194 and miR-5100 decreased in MSCs compared with parent cells . In highly metastatic PANC-1 cells(MPCs), miR-1247-3p, miR-2964a-5p and miR-3135b increased and miR-377-5p, miR-4454 and miR-5100 decreased in MPCs compared with parent cells. We have identified several miRNAs from pancreatic stellate cell, highly metastatic pancreatic cancer cells and IPMN that has possibility of becoming markers for prognostic value of pancreatic cancer.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Elevated Expression Level of MicroRNA-196a Is Predictive of Intestinal-Type Intraductal Papillary Mucinous Neoplasm of the Pancreas2013
Author(s)
Aso T, Ohtsuka T, Tamura K, Ideno N, Kono H, Nagayoshi Y, Ohuchida K, Ueda J, Takahata S, Shindo K, Aishima S, Oda Y, Mizumoto K, Tanaka M.
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Journal Title
Pancreas
Volume: 43(3)
Pages: 361-366
Related Report
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[Journal Article] MAL2 expression predicts distant metastasis and short survival in pancreatic cancer2013
Author(s)
Daiki Eguchi, Kenoki Ohuchida, Shingo Kozono, Naoki Ikenaga, Koji Shindo, Lin Cui, Kenji Fujiwara, Shin Akagawa, Takao Ohtsuka, Shunichi Takahata, Shoji Tokunaga, Kazuhiro Mizumoto, Masao Tanaka
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Journal Title
Surgery
Volume: 154(3)
Pages: 573-82
Related Report
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[Journal Article] CD271 subpopulation of pancreatic stellate cells correlates with prognosis of pancreatic cancer and is regulated by interaction with cancer cells
Author(s)
Fujiwara K, Ohuchida K, Mizumoto K, Shindo K, Eguchi D, Kozono S, Ikenaga N, Ohtsuka T, Takahata S, Aishima S, Tanaka M.
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Related Report
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